[Histonet] Paraffins for mouse tissue

[Gayle Callis]

No one is superior, in my opinion, having tried just about everything on 
the market over the years.   We have used Surgipath's infiltration media 
and their embedding media combination/system with great success.  We have 
gone back to Fishers Tissue Prep 2, consensus of opinion for mouse and 
hamster brains, decalcified mouse bones, and all other murine tissues, we 
like it best for both infiltration and embedding.  We are using up Tissue 
Prep from Fisher and find it bit softer but adequate for infiltration and 
embedding.   In general, once we chose a paraffin, we use it for both 
infiltration and embedding with the exception of Surgipaths tidy combo 
system.

I think the key is how mouse tissue is processed before the paraffin 
infiltration/embedding, if not done optimally, no paraffin has bailed us 
out of trouble nor gives us an opinion on what is best.


At 11:36 AM 10/28/2005, you wrote:
>What are the best paraffins to use for infiltrating and embedding mouse 
>tissues. I currently use one for infiltrating and another for embedding 
>but I would love to see what others are using and recommend to be the best.
>
>Thanks,
>Andrea
>--
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)