[Histonet] RNase contamination during in-situ

[Edmondson David (RBV) NHS Christie Tr]

30% Hydrogen peroxide diluted 1 in 10, for ten minutes and rinse glassware with Methanol,,, was what we were advised to use and the slides appear to be not a problem.     Do folks agree??
David
Manchester, UK
The reagents in the kit should not be a problem.

The wash/diluent water could be double distilled but the high grade supply for the Bayer analyser in Biochem seems fine


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Barnhart,
Tammy
Sent: 28 October 2005 16:31
To: Histonet (E-mail)
Subject: [Histonet] RNase contamination during in-situ


I need some help...we are going to try using the Dako PNA ISH kit for
Kappa/Lambda and are concerned about the RNase contamination issue.  Can
anyone give me an idea as to what product to use to take care of this on
surfaces, glassware and reagents?  Any and all suggestions would be
appreciated.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND



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