[Histonet] questions about rhodanine-copper stain

[Gudrun Lang]

I am dealing with the rhodanine stain, because we shall establish it in our
histolab. 

I've read three different protocols (AFIP, Churukian, webpath). 

 

AFIP and Churukian require sodiumacetat solutions to mix with the
stocksolution, 

the others require just the dilution of the stocksolution in Aqua dest.

Why? Do both versions work?

 

Churukian demands coverslipping with aqueous medium, the others don't.

Is the staining result soluble in ethanol?

 

The stability of the 0,2% rhodanine/ethanol stocksolution is described
between one day and three months.

What is the right shelf live?

 

I hope someone can explain it to me. 

 

 

Gudrun Lang

 

General hospital Linz, Austria