[Histonet] DISSECT AID

Mon Oct 24 13:06:33 CDT 2005

WE are using the Dissect Aid and tow of my pathologists really like it.
Terri Clarke
Histology Supervisor
All Saints Laboratory
3801 Spring Street
Racine, Wi 53405
mclarke <@t> allsaintshealthcare.org
262-687-6609

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Subject: Histonet Digest, Vol 23, Issue 27

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Today's Topics:

   1. Disect-Aid (BSylinda <@t> aol.com)
   2. Re: Disect-Aid (Rene J Buesa)
   3. Re: Disect-Aid (Krat18 <@t> aol.com)
   4. Re: Disect-Aid (John Kiernan)

----------------------------------------------------------------------

Message: 1
Date: Sat, 22 Oct 2005 16:35:42 EDT
From: BSylinda <@t> aol.com
Subject: [Histonet] Disect-Aid
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1a6.422d78da.308bfc9e <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Hi Histoland,
    I am writing for more information about  disect-aid.  Do anyone
have any 
experience in using this reagent for  fatty tissue such as lymph node
tissue. 
 Out pathologist is interested in  trying this.  Any feedback of pros
and 
cons of using this product would be  greatly appreciated.  Could you
also forward 
vendor information as  well.

Thanks,
Sylinda

------------------------------

Message: 2
Date: Sat, 22 Oct 2005 15:12:26 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Disect-Aid
To: BSylinda <@t> aol.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051022221226.58568.qmail <@t> web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Sylinda:
We used for lymph nodes the following solution:
100% ethanol ------2 L + Distilled water -----0.68 L + 37% formaldehyde
---0.32 L + acetic acid--0.2 L (for a total of 3.20 L).
It worked very well and even the smallests lymph nodes could be
detected.
Rene J.

BSylinda <@t> aol.com wrote:
Hi Histoland,
I am writing for more information about disect-aid. Do anyone have any 
experience in using this reagent for fatty tissue such as lymph node
tissue. 
Out pathologist is interested in trying this. Any feedback of pros and 
cons of using this product would be greatly appreciated. Could you also
forward 
vendor information as well.

Thanks,
Sylinda

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Message: 3
Date: Sat, 22 Oct 2005 22:34:41 EDT
From: Krat18 <@t> aol.com
Subject: Re: [Histonet] Disect-Aid
To: BSylinda <@t> aol.com, histonet <@t> lists.utsouthwestern.edu
Message-ID: <146.4f5550fc.308c50c1 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Sylinda,

Our PA swears by Dissect Aid ...... she uses it at both hospitals where
she  
works.   She slices the tissue into thick slices and just covers it
with 
Dissect-Aid, and after an hour or so, the lymph nodes turn white and
become  very 
easy to spot.

Dissect Aid is a product of the Decal Chemical Corporation:
        Please take a look at  Dissect Aid on our website, 
www.decal-bone.com.  It  is 
a special fixative  for revealing lymph nodes which we have  been 
manufacturing for  over 20 years. You can call our toll free number or
reply 
to this email for  a free  sample.

.            Cliff  Berger
Decal  Chemical  Corp
1800-428-5856
_www.decal-bone.com_ (http://www.decal-bone.com) 

I hope this helps you!

Karen Raterman
St. Mary's Health Center
St. Louis, MO 63117
_krat18 <@t> aol.com_ (mailto:krat18 <@t> aol.com) 

------------------------------

Message: 4
Date: Sun, 23 Oct 2005 00:01:55 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Disect-Aid
To: BSylinda <@t> aol.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <435B0B33.3718159A <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii

Numerous Histonet messages have extolled Davidson's fixative
for this purpose. It contains less alcohol than Rene Buesa's
mixture, so it's cheaper! The recipe is on Dr Bob Richmond's
web site:
http://members.aol.com/RSRICHMOND/histology.html

For fun, and just to be pedantic: the word is dissect, and its
first sy

llable rhymes with hiss, not with dice. The origin is from sect
(cut) and dis (apart). 

Until about 15 years ago it didn't matter how you pronounced the
word, but there is now an important  stereological technique
called "the disector" (only one s) that obtains unbiased
estimates of numbers and volumes of cells and other objects. The
method involves collecting measurements from two (di) sections
through a specimen. A dissector must obtain the specimen, and a
histotechnologist (a sector who uses dyes) must prepare it for a
morphometrist to apply the disector. The disector's results are
tables of numbers, split up into many sections and very difficult
to understand.

We who dissect should proudly defend our skills by saying the
word correctly. Dye-sect (for dissect) is in the same class
(Homer Simpson) as nucular (for nuclear). Words like bacteria,
data and media (all plural) are often used in newspapers as if
they were singular rather than plural. 

John Kiernan
Anatomy, UWO
London, Canada.
__________________________________________
BSylinda <@t> aol.com wrote:
> 
> Hi Histoland,
>     I am writing for more information about  disect-aid.  Do anyone
have any
> experience in using this reagent for  fatty tissue such as lymph node
tissue.
>  Out pathologist is interested in  trying this.  Any feedback of pros
and
> cons of using this product would be  greatly appreciated.  Could you
also forward
> vendor information as  well.
> 
> Thanks,
> Sylinda
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

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End of Histonet Digest, Vol 23, Issue 27
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