[Histonet] Block cutting protocols

Nancy Lemke nsnwl <@t> neuro.hfh.edu
Mon Oct 24 09:52:07 CDT 2005


This is an absolute NEVER.  It is similar to embedding and  simultaneously opening cassettes of tissue with like specimens but different cases. Everybody knows about tissue bits flying out of the cassette when the top is removed. That is an absolute recipe for disaster.  The multiple blocks from the same case on the waterbath at once is fine, of course, but different cases.......SCARY AS HALLOWEEN!
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Gayle Callis" gcallis <@t> montana.edu
Date: Mon, 24 Oct 2005 10:45:25 -0400
To: "Joe Nocito" jnocito <@t> pathreflab.com
Subject: RE: [Histonet] Block cutting protocols

> Never!  Hmm, this could be a legal tissue issue, if you mix up a case or 
> pick up the bad guys (cells as floaters) with onto a slide with next case 
> negative good guys and the good guy gets the WRONG diagnosis, you may be 
> seeing your attorney.  Worked in a lab where this was forbidden, and we 
> carefully skimmed the top of the waterbath between cases with a kimwipe to 
> pick up last ribbon garbage.   There is always a chance you may have a cell 
> you don't want, but we tried to minimize the chances of this happening 
> between all cases.
> 
> 
> 
> At 02:59 PM 10/21/2005, you wrote:
> >Susan,
> >Only if you are looking for trouble. I say no
> >
> >Joe Nocito
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molinari,
> >Betsy
> >Sent: Tuesday, October 18, 2005 5:44 AM
> >To: histonet <@t> lists.utsouthwestern.edu
> >Subject: RE: [Histonet] Block cutting protocols
> >
> >No
> >
> >Betsy Molinari HT (ASCP)
> >Texas Heart Institute
> >Cardiovascular Pathology
> >6770 Bertner Ave.
> >Houston,TX 77030
> >832-355-6524
> >832-355-6812 (fax)
> >
> >
> >-----Original Message-----
> >From: histonet-bounces <@t> lists.utsouthwestern.edu
> >[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> >Histology SLU
> >Sent: Monday, October 17, 2005 9:05 AM
> >To: histonet <@t> lists.utsouthwestern.edu
> >Subject: [Histonet] Block cutting protocols
> >
> >Hello All:
> >
> >I hope that you all won't "flame" me with this question.  I do not want
> >to offer any information yet as to why I am asking.  I want your
> >responses to be unbiased by the circumstances for which I am asking.
> >
> >The question:
> >
> >Do any of you techs cutting clinical paraffin embedded blocks, lay out
> >multiple ribbons from multiple blocks on your water bath at one time and
> >then pick up your sections for each block?
> >
> >I would like tech and supervisor responses please.  Thanks so much.  You
> >guys are always such a help.
> >
> >Susan
> >
> >
> >---------------------------------
> >  Yahoo! Music Unlimited - Access over 1 million songs. Try it free.
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> >
> >
> >_______________________________________________
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> 
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> 
> 
> 
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> 


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