Fwd: RE: [Histonet] Ventana immunostainer

Janice A Mahoney jmahoney <@t> alegent.org
Wed Oct 19 10:33:22 CDT 2005

I use Ventana benchmarks and the XT in my lab and have absolutely no problems.  We have beautiful reproducible staining with every antibody we have worked up. 
Paula is the person who is having trouble with some antibodies.
I think it is very important when bring up a new instrument or system that you eliminate any variables you can.  I'm sure Ventana will give you some references of happy customers.  Find out what a lab who has good consistent results uses as far a water, slides, fixation times, ptotocols etc. 
Most problems can be overcome from that point on with a little tweaking of diluents,  cell conditioning, etc.
Eliminating the variables on your controls is also important.  
Once you get antibodies up and running I think you will be very happy with Ventana's quality and reproducibility.  
Have your rep check the instrument for any instrument problems like pads not heating properly.
Good Luck
Jan Mahoney

>>> "Janice A Mahoney" <jmahoney <@t> alegent.org> 10/19/2005 9:32 AM >>>

>>> "Cazares, Ruth" <RCazares <@t> schosp.org> 10/19/2005 9:30 AM >>>

I had a similar problem when we first got our Ventana instrument. What
it turned out to be the slides. We ordered from a cheaper source and got
spotty staining.  We switched to the Fisher (+) slides and have not had
a problem for the last 8 months.

Good Luck!

Ruth Cazares
Swedish Covenant Hospital
Chicago, IL

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Janice A
Sent: Tuesday, October 18, 2005 8:17 PM
To: histo20 <@t> hotmail.com; histonet <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] Ventana immunostainer

What kind of water are you using to make your solutions?

>>> "Paula Wilder" <histo20 <@t> hotmail.com> 10/18/2005 6:12 PM >>>
Our institution is trying the new Ventana immunostainer.  We have had
unit since July and everything was staining nicely.  Then we started to
variable staining, especially with CK20, ER and PR, ki67.  Ventana reps
been working diligently to get this problem resolved.  We are using 
pre-dilutes and the staining seems to be fine the first time, then 
increasingly variable.  The reps took our controls to another area
and tested them there and the staining was fine; hence, the problem is
our machine.  I know they will replace this instrument if it continues
fail, but I was wondering if anyone out there has similar problems.
for any help!

Paula Wilder
St. Joseph Medical Center
7601 Osler Drive
Towson, MD 21204

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