[Histonet] RE: Nissl Staining on Thick Sections

[Melissa Gonzalez]

Paul, have you tried Molecular Probes? Their Nissl stains work great, you just have to adj. [ ]'s to your needs. Super fast and easy, and very  bright specific results.  

Melissa
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Message: 2
Date: Tue, 18 Oct 2005 11:34:24 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: [Histonet] Nissl Staining on Thick Sections
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
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	<09C945920A6B654199F7A58A1D7D1FDE017175F8 <@t> lsexch.lsmaster.lifespan.org>
	
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Does anyone routinely do Nissl staining on 30 to 40 micron frozen sections?
That's what the current project calls for, and I am finding that Nissl
methods designed for 6 to 8 micron sections don't work well on the thick
sections. They don't differentiate properly.  If anyone has a Nissl protocol
that works well on such thick sections, I would be grateful for any
information.

Paul M.