[Histonet] Immunohistochemistry questions

Andrea Hooper anh2006 <@t> med.cornell.edu
Mon Oct 17 07:27:46 CDT 2005

If you look at your sections with no primary and no secondary you should be able to determine if it is 
autofluorescence or not. It is my experience that paraffin embedded bone is highly autofluorescent and needs to 
be blocked prior to staining. 

If you provide more details of your protocol perhaps we can help.

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