[Histonet] Immunohistochemistry questions

[pex]

Dear all,
 
I am confused about immunohistochemistry. I have had some nice results in immunohistochemistry of paraffin-embedded bone and cartilage sections with alkaline phosphatase antibody. But there are not some good results in bone sections if I do immunofluorescence with the same antibody, I do not know the reasons. Maybe due to too much autofluorescence, but I am not sure.
Any suggestions will be helpful. Thanks a lot.
 
Guofeng 

		
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