[Histonet] cartilage staining

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Oct 15 10:02:34 CDT 2005

Toluidine blue has always worked better for me. Both have a similar chemical composition
which for Safranin-O is C20 H19 N4 Cl (MW=351) and for Toluidene Blue is C15 H16 N3 Cl and S (MW=238).
Their difference is not in the fact that Toluidine Blue has a smaller mollecular weight, but
because Toluidine Blue has Sulfur in its mollecule. Probably this is the reason for
a better affinity with the Sulfur present in cartilage.
Rene J.

Beppe Intini <gintini <@t> buffalo.edu> wrote:
Dear Histo-Friends,
I hope you can help me again....!
Just a quick question: is Safranin-O better than Toluidine blue for
cartilage staining? And Why?
Beppe Intini
Giuseppe Intini
Clinical Assistant Professor - Periodontics
PhD Candidate - Oral Biology
University at Buffalo
Buffalo, NY 14214

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