[Histonet] Dry tissue

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Oct 14 12:59:19 CDT 2005

If nothing has changed in your processing routine then you should look at the biopsies and how they are collected. Once we had a similar problem and it turned out that the biopsies were not fixed immediately they were collected (they dried out before being fixed). 
Also, are the same fixative as before being used?
Have you had any changes in the reagents suppliers?
Additionally all our "dryness" problems were eliminated when we start using a mixture of ethanol/isopropanol/mineral oil which allowed us to start dehydration/infiltration steps at a time ratio of 4.8:1 with relation with the initial dehydration.
Just a thought!
Rene J.

"Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
I thought I knew most of the processing answers when hit with
problematic tissues to cut, but, lately, I am stymied!! Lately, our
smaller biopsies have been very dry and nothing has changed in our
processing routine or reagents. I did lessen the times in absolute a
little and also let the time in xylene be lessened. I am not seeing the
change I thought I would. Can anyone give me any suggestions as to how
to process small biopsies in the same processor with large specimens
without drying out the small GI biopsies and still getting the proper
processing on large speicmens. Thanks ahead of time for responding to
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.

If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

 Yahoo! Music Unlimited - Access over 1 million songs. Try it free.

More information about the Histonet mailing list