[Histonet] Height adjustable tables
Tim Webster
twebster <@t> nmcinc.org
Fri Oct 14 09:05:18 CDT 2005
Hi Bonnie,
We are presently ordering three adjustable work tables from a company called
Bostontec. (Bob Doucette 508-574 1619) Also check out Bostontec.com for
images of their installations. The tables are electrically adjustable over a
wide range as we have technologists ranging from 5'2" to 6'1 in height.
We looked at various vendors, but settled on Bostontec after considering
pricing and design flexibility. Having said that, we haven't used the tables
yet, so this is no way an endorsement blah blah blah - insert liability
disclaimer here.
However, they have been very easy to deal with.
Have a great day and good luck.
Tim
Tim Webster
Histology Specialist
Northwestern Medical Center
133 Fairfield Street
St Albans, VT 05478
(802) 524-1070 x4349 (Dept. & Voice mail)
twebster <@t> nmcinc.org
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, October 13, 2005 1:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 23, Issue 14
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Today's Topics:
1. RE: use of microwaves (Pixley, Sarah (pixleysk))
2. Re: Air Bubbles in Manual Coverslipping (Rene J Buesa)
3. RE: remove grinding debris (Rittman, Barry R)
4. slide, cassette labelers (Osborn, Sharon)
5. RE: slide, cassette labelers (Schumacher, Jennifer J)
6. Re: An old tissue processor (rchiovetti <@t> aol.com)
7. Coverslipping technics (Osborn, Sharon)
8. Re: RE: use of microwaves (Rene J Buesa)
9. Re: Cytologies crossing state lines (Rene J Buesa)
10. mounting media for EGFP in liver (Caroline Bass)
11. RE: Cytologies crossing state lines (Ford Royer)
12. decalcifying solutions for skeletochronology (Malcolm McCallum)
13. Re: decalcifying solutions for skeletochronology (John Kiernan)
14. Histotech Opportunites in your Area (Eric Dye (ext 223))
15. Height Adjustable tables (Bonnie J. McMahill)
16. SGLT1 antibody (Jonathan Wilson)
17. RE: beta-2- microglobulin (Edwards, R.E.)
18. Re: decalcifying solutions for skeletochronology (Rene J Buesa)
19. MOUSE BRAIN FIXATION (Adam Perry)
20. RE: MOUSE BRAIN FIXATION (Favara, Cynthia (NIH/NIAID))
21. Re: Tissue with Fat (Arshia Mian)
----------------------------------------------------------------------
Message: 1
Date: Wed, 12 Oct 2005 13:20:52 -0400
From: "Pixley, Sarah \(pixleysk\)" <PIXLEYSK <@t> UCMAIL.UC.EDU>
Subject: [Histonet] RE: use of microwaves
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E7340445E0595A418B2F8DE0AE480E200364CD53 <@t> ucmail6.ad.uc.edu>
Content-Type: text/plain; charset="us-ascii"
Dear All:
I wondered if anyone that is currently using microwaves to heat samples
has considered using a sand dry-bath for heating? The molecular
biologists have all switched to those and they are really great. They
are just heaters with a well that you put a good quality sand in. They
can heat the sand up to 130 degrees centigrade. Then you stick your
sample in the sand. The heat is very consistent. You can have something
go to close to boiling almost instantly, without the muss and fuss of a
hot water bath (or a microwave). Aside from a few bits of sand getting
out, they are very civilized and clean. And they take up FAR less
counter space than a microwave. Just a thought, but it is from someone
who is not doing HIER.
Sarah
------------------------------
Message: 2
Date: Wed, 12 Oct 2005 10:22:58 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Air Bubbles in Manual Coverslipping
To: Travis Troyer <ttroyer <@t> petersonlab.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051012172258.72021.qmail <@t> web61225.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
You should start with the fluidity of the "glue" (I always prefer to call it
"mounting medium").
It should be fluid enough to spread over the section. You should apply a very
small drop over the section with either a plastic dropper or a wood
applicator.
The coverslip should be over a paper towel and you should get the
slide/section with the mounting medium drop on it in contact with the
coverslip and press it gently.
You should wrap your finger with a cotton fiber disposable gauze to
immediately clean the excess mounting medium.
If the mounting medium is liquid enough you will prevent the bubbles, and
even if the amount is in excess you can clean it around the coverslip
eliminating the "overflow".
Rest the coverslipped slide flat to dry out.
Dammar resin or any other commercial mounting medium will do. Regardless if
the diluent is toluene or xylene, you can always adjust the fluidity with
xylene (which is totally miscible with toluene).
Practice is essential ("like getting to Carnegie Hall!").
Hope this will help.
Rene J.
Travis Troyer <ttroyer <@t> petersonlab.com> wrote:
We have recently come under the "gun" for excess bubbles and glue on the
slides after coverslipping. We currently do all of coverslipping manually and
was wondering if anyone had any helpful hints to keep the bubbles out and and
excess glue off the slide.
Thanks for the assistance,
Travis Troyer
Peterson Laboratory Services
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Message: 3
Date: Wed, 12 Oct 2005 13:18:31 -0500
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: RE: [Histonet] remove grinding debris
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EA1FDD2A141B7448B4B1AFFFCAC08DE404B6D011 <@t> UTHEVS1.mail.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"
Myriam.
Are you using water as a lubricant on the paper? If so remember that
Epon is somewhat hygroscopic. Can grind using a neutral oil.
If grinding dry then repeat the grinding on a frosted glass plate. This
often will remove a lot of the debris. Can first try a commercial
frosted slide or prepare your own using alumina.
Are you sure that the debris is not being introduced during staining?
Hydroxyapatite and other minerals can cause precipitates and also may
bind to some dyes.
Barry
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Myri37 <@t> aol.com
Sent: Wednesday, October 12, 2005 10:56 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] remove grinding debris
Hello everyone,
I grind on abrasive sandpaper, sections of titanium implants embedded in
epon. Titanium is coated with a hydroxyapatite layer and soft tissue.
After staining with RBS (rapid bone stain), i see on slides a lot of
debris,i think they are grinding debris and titanium or hydroxyapatite
dust...
Does anyone have a process to remove these debris ?
Do you recommend a wash with a specific detergent solution after
grinding, does 0.1 % Zephiran chloride solution efficient ?
Thank you very much for any advice.
Myriam
NI France
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------------------------------
Message: 4
Date: Wed, 12 Oct 2005 14:49:25 -0400
From: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>
Subject: [Histonet] slide, cassette labelers
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29B25753F6B1D51196110002A589D44402398230 <@t> PALMSG30.us.schp.com>
Content-Type: text/plain
Jill,
Please consider the Leica cassette and slide printers (not
labelers). We have had ours almost 1 year with multiple users. They are
very good, consistent and the service has been great. They are just about
ready for a major upgrade to solve some problems that developed this past
year with units out in the field. Still with those problems, these are
better than the previous ones. As you, I have used the ShurMark for
years...it was great for its time and definitely filled a void. However,
the Leica is the new generation of labelers and well worth your having it
demonstrated in your facility.
Sharon Osborn
DNAX, Schering Plough BioPharma
Palo Alto, CA
650.496.6539
Date: Tue, 11 Oct 2005 12:23:42 -0700 (PDT)
From: Jill Cox <jcox90 <@t> yahoo.com>
Subject: [Histonet] Cassette Labeler
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051011192343.43419.qmail <@t> web52111.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello Histonetters,
We will be purchasing a cassette labeler soon and hopefully slide labeler
down the road. I would love your feedback on which one's are more reliable.
I have used a Shurmark in the past and it seemed to work fine. If there is
something else out there you are using I would like to look into that as
well. Thank you in advance, Jill
Jill Cox HT (ASCP)
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Message: 5
Date: Wed, 12 Oct 2005 14:02:22 -0500
From: "Schumacher, Jennifer J" <JSCHUMA1 <@t> Fairview.org>
Subject: RE: [Histonet] slide, cassette labelers
To: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BC4B98C5FDACBC4B90961774B549B00DA99F5E <@t> digsmxmbx03.Fairview.org>
Content-Type: text/plain; charset="us-ascii"
I agree completely. Our slide labeler was down one day, and we tried to
use our old ShurMark as a backup. Only then did we remember how lucky
we were to have the new units. They are cleaner, easier to use, and oh
so FAST!!! Plus, we are saving a fortune by not buying the "colormark"
slides any longer. I would highly recommend them. Jennifer Schumacher,
University of Minnesota Medical Center, Fairview
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Osborn,
Sharon
Sent: Wednesday, October 12, 2005 1:49 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] slide, cassette labelers
Jill,
Please consider the Leica cassette and slide printers (not
labelers). We have had ours almost 1 year with multiple users. They
are very good, consistent and the service has been great. They are just
about ready for a major upgrade to solve some problems that developed
this past year with units out in the field. Still with those problems,
these are better than the previous ones. As you, I have used the
ShurMark for years...it was great for its time and definitely filled a
void. However, the Leica is the new generation of labelers and well
worth your having it demonstrated in your facility.
Sharon Osborn
DNAX, Schering Plough BioPharma
Palo Alto, CA
650.496.6539
Date: Tue, 11 Oct 2005 12:23:42 -0700 (PDT)
From: Jill Cox <jcox90 <@t> yahoo.com>
Subject: [Histonet] Cassette Labeler
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051011192343.43419.qmail <@t> web52111.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello Histonetters,
We will be purchasing a cassette labeler soon and hopefully slide
labeler down the road. I would love your feedback on which one's are
more reliable.
I have used a Shurmark in the past and it seemed to work fine. If there
is something else out there you are using I would like to look into that
as well. Thank you in advance, Jill
Jill Cox HT (ASCP)
*********************************************************************
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-- Please immediately and permanently delete.
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------------------------------
Message: 6
Date: Wed, 12 Oct 2005 15:03:44 -0400
From: rchiovetti <@t> aol.com
Subject: Re: [Histonet] An old tissue processor
To: pruegg <@t> ihctech.net, histonet <@t> pathology.swmed.edu
Message-ID: <8C79D76CD48E79F-17C4-3A40 <@t> MBLK-M07.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Patsy,
Boy that *is* an oldie! The Fisher products were taken over by RMC, Inc.
several years ago. Then RMC sold the product line to Ventana Medical Systems
here in Tucson.
It's a long shot, but you might try calling Ventana at (800) 227-2155. Ask
for Tom Kennedy. Tom's was with the product line from the original Fisher
days, then RMC, then Ventana. He would know if anyone would about parts for
the 166.
Good Luck!
Cheers,
Bob Chiovetti
The Microscope works
Arizona's Microscopy Resource
Tucson, Arizona USA
Tel./Fax 520-546-4986
Member, Arizona Small Business Association
(www.asba.com)
-----Original Message-----
From: Patsy Ruegg <pruegg <@t> ihctech.net>
To: histonet <@t> pathology.swmed.edu
Sent: Wed, 12 Oct 2005 10:03:36 -0600
Subject: [Histonet] An old tissue processor
We have an old Fisher Histomatic Model 166A tissue processor that is on the
fritz. Does anyone know where we might be able to find parts and service
for such a critter????
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/>
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------------------------------
Message: 7
Date: Wed, 12 Oct 2005 15:03:52 -0400
From: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>
Subject: [Histonet] Coverslipping technics
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29B25753F6B1D51196110002A589D44402398231 <@t> PALMSG30.us.schp.com>
Content-Type: text/plain
Travis,
One of my favorite tasks is coverslipping. However, due to pressing
duties and volume, the automated coverslipper does the work. To get a good
coverslip technic is essential when doing so manually. This is the one I
use:
1. The slide is moist from the xylene. I use a dropper (I do have
one of the old type bottles with the little glass dropper with a round glass
bulb on its end)or a wooden applicator stick. Dip the stick into xylene to
moisten it then into the mounting medium. The mounting medium will form a
ball drop on the applicator.
2. Place the ball drop of mounting medium directly on the lower
edge of the slide. I generally make a little line of it along the edge.
3. Pick up the coverslip with two fingers(I use thumb and index)
along the coverslip edges. Be certain there are no sticky coverslips stuck
together. Place the long edge of the coverslip along the lower edge of the
slide on the mounting medium. The slide will gently lay down on the
mounting medium and move it over the slide as it makes contact. There are
rarely any bubbles.
4. If there are bubbles, gentle pressure with forceps or the
applicator end (not one with mounting medium) will move those out. Also,
there is rarely any excell medium around the edges. I
5. If there is excess mountimg medium around the edges, wipe it
gently with a Kimwipe moistened with xylene or use a #3 artist brush (camel
hair) dipped in xylene to clean the edges of the slide. Lay in mats to dry.
Practise will develop this technic such that you will be faster than
the automated coverslipper--the glass ones--which are all I recommend!..:-)
Sharon Osborn
DNAX, Schering Plough BioPharma
Palo Alto, CA
650.496.6539
Date: Wed, 12 Oct 2005 11:58:11 -0500
From: "Travis Troyer" <ttroyer <@t> petersonlab.com>
Subject: [Histonet] Air Bubbles in Manual Coverslipping
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003501c5cf4e$217bb000$6601010a <@t> Peterson.local>
Content-Type: text/plain; charset="iso-8859-1"
We have recently come under the "gun" for excess bubbles and glue on the
slides after coverslipping. We currently do all of coverslipping manually
and was wondering if anyone had any helpful hints to keep the bubbles out
and and excess glue off the slide. Thanks for the assistance, Travis Troyer
Peterson Laboratory Services
*********************************************************************
This message and any attachments are solely for the intended recipient. If
you are not the intended recipient, disclosure, copying, use or distribution
of the information included in this message is prohibited -- Please
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------------------------------
Message: 8
Date: Wed, 12 Oct 2005 13:45:52 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] RE: use of microwaves
To: "Pixley, Sarah \(pixleysk\)" <PIXLEYSK <@t> UCMAIL.UC.EDU>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051012204552.15123.qmail <@t> web61225.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I have used sand bath during some biochemistry procedures and I agree with
you that they are great BUT (there is always a BUT) temperature regulation is
somewhat tricky and will depend on the amount of sand and the condition of
the heating elements.
For HIER you will have to consider the following aspects:
1- it requires almost one half an hour to complete.
2- How much HIER solution are you going to use? If not enough it will
probably evaporate
while in the sand bath after just a few minutes.
3- evaporation is prevented in the steamer because the environment is water
vapor saturated
and the vapor phase is, lets say "compensated".
4- in the microwave oven you will limit evaporation because HIER is quicker
(due to the effect of the microwaves on the bipolar molecules within the
tissue, and this, along with heat, is
the fundament for the microwave oven).
You will have to do many tests to find out an ideal ratio
volume/temperatureXtime for a HIER done in a sand bath.
The steamer is so simple to operate, and so consistent, that I personally
would not try
the sand bath to do HIER.
This is just my opinion. Hope this will help!
Rene J.
"Pixley, Sarah (pixleysk)" <PIXLEYSK <@t> UCMAIL.UC.EDU> wrote:
Dear All:
I wondered if anyone that is currently using microwaves to heat samples
has considered using a sand dry-bath for heating? The molecular
biologists have all switched to those and they are really great. They
are just heaters with a well that you put a good quality sand in. They
can heat the sand up to 130 degrees centigrade. Then you stick your
sample in the sand. The heat is very consistent. You can have something
go to close to boiling almost instantly, without the muss and fuss of a
hot water bath (or a microwave). Aside from a few bits of sand getting
out, they are very civilized and clean. And they take up FAR less
counter space than a microwave. Just a thought, but it is from someone
who is not doing HIER.
Sarah
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Message: 9
Date: Wed, 12 Oct 2005 13:49:14 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Cytologies crossing state lines
To: "Michelle D. Moore" <tmhpath <@t> amigo.net>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20051012204914.21514.qmail <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I would contact UPS or FederalExpress. Some time ago we had to send some
samples and they knew all the regulations/limitations (since they are the
carriers).
Rene J.
"Michelle D. Moore" <tmhpath <@t> amigo.net> wrote:
Hello in histoland. I am looking for information on whether or not you can
ship freshly collected pap smears across state lines?! I have been told you
can by one place and then told that you cannot at all. I know that you cannot
send them across California's state line but what about the rest of the
nation? I appreciate any help I can get. Thank you for your time and help.
Michelle D. Moore
The Memorial Hospital
Craig, CO
tmhpath <@t> amigo.net
_______________________________________________
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Message: 10
Date: Wed, 12 Oct 2005 17:19:07 -0400
From: Caroline Bass <cbass <@t> bidmc.harvard.edu>
Subject: [Histonet] mounting media for EGFP in liver
To: Histonet (E-mail) <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <41489ADD-7323-463D-88F8-42BBAC1B341A <@t> bidmc.harvard.edu>
Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed
Hello,
I have used a viral vector to transduce liver and pancreatic tissues
in the mouse with EGFP and RFP. I have cut portions of the liver and
pancreas into small blocks and fixed overnight with NBF. What is the
best way to visualize the signal? I want to cut 30 micron sections,
as I only have access to a sliding microtome. Could someone
recommend a good, and hopefully inexpensive, mounting media for
fluorescence? I have a fairly strong signal but I am worried about
losing it if I use the wrong product. I am not very experienced with
fluorescence so I really don't know what to use.
Someone recommended Aqua PolyMount from polysciences.
Any suggestions would be appreciated.
Caroline
------------------------------
Message: 11
Date: Wed, 12 Oct 2005 16:19:09 -0500
From: "Ford Royer" <froyer <@t> bitstream.net>
Subject: RE: [Histonet] Cytologies crossing state lines
To: "Michelle D. Moore" <tmhpath <@t> amigo.net>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <PMEKIGLGBNOANKKFKOOCIEKFCAAA.froyer <@t> bitstream.net>
Content-Type: text/plain; charset="iso-8859-1"
I believe that it is okay, as long as it is not for an immoral purpose...
...sorry, couldn't resist. ;-)
(For you youngsters out there... see "The Mann Act of 1910")
~ Ford
Ford Royer, MT(ASCP)
Minnesota Medical Specialists
7177 Madison Ave. W.
Golden Valley, MN 55427-3601
888-790-9686
<froyer <@t> bitstream.net>
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Michelle D.
Moore
Sent: Wednesday, October 12, 2005 11:50 AM
To: Histonet
Subject: [Histonet] Cytologies crossing state lines
Hello in histoland. I am looking for information on whether or not you can
ship freshly collected pap smears across state lines?! I have been told you
can by one place and then told that you cannot at all. I know that you
cannot send them across California's state line but what about the rest of
the nation? I appreciate any help I can get. Thank you for your time and
help.
Michelle D. Moore
The Memorial Hospital
Craig, CO
tmhpath <@t> amigo.net
_______________________________________________
Histonet mailing list
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------------------------------
Message: 12
Date: Wed, 12 Oct 2005 23:01:16 -0500
From: "Malcolm McCallum" <Malcolm.McCallum <@t> tamut.edu>
Subject: [Histonet] decalcifying solutions for skeletochronology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EFF133CED606184A8FC9A6FBBF46714E013C903A <@t> STAN.tamut.local>
Content-Type: text/plain; charset="iso-8859-1"
Hi,
I am having some students do various skeletochronology projects with
amphibians and reptiles. I could just buy some decalcifying solution, but we
have so many acids, I figured I would make some. Any good recipes out there
for decalcifying bone w/o heating?
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
------------------------------
Message: 13
Date: Thu, 13 Oct 2005 00:57:47 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] decalcifying solutions for skeletochronology
To: Malcolm McCallum <Malcolm.McCallum <@t> tamut.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <434DE94B.61DC3E1C <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii
Dear Dr McCallum,
Every book in the field of histotechnology contains
several recipes for decalcifying. The calcified material
can be removed by dissolving in a suitable acid (usually
hydrochloric or formic) or by chelation with a suitable
anion (usually citrate or EDTA). The choice of method is
based on the requirements of the investigation.
If you are in doubt about choosing a method, ask the
internet for references rather than recipes.
There are scores of books and hundreds of papers!
A solid place to start searxhing the literature is
Pearse's Histochemistry.
John A. Kiernan MB, ChB, PhD, DSc
Professor, Dept of Anatomy & Cell Biology
The University of Western Ontario
London, Canada.
----------------------------
Malcolm McCallum wrote:
>
> Hi,
> I am having some students do various skeletochronology projects with
amphibians and reptiles. I could just buy some decalcifying solution, but we
have so many acids, I figured I would make some. Any good recipes out there
for decalcifying bone w/o heating?
>
> Malcolm L. McCallum
> Assistant Professor
> Department of Biological Sciences
> Texas A&M University Texarkana
> 2600 Robison Rd.
> Texarkana, TX 75501
> O: 1-903-233-3134
> H: 1-903-791-3843
> Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
>
------------------------------
Message: 14
Date: Wed, 12 Oct 2005 20:58:54 -0400
From: Eric Dye (ext 223) <Eric <@t> ategra.com>
Subject: [Histonet] Histotech Opportunites in your Area
To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <QkZUNEJBTiZFOSVCIVVgMTM0NDM2NTY <@t> blkdell3l>
Content-Type: text/plain
Histonetters
I'm presently on a search for my best client companies seeking HistoTech
Supervisors and Histo Bench Techs. These are mostly fulltime permanent
positions. (I have a few travel/temp HistoTech positions as well - see
below). Most of my Histo Techs positions are clinical, although I do have a
few Research Histo Tech positions as well.
Here are some of my Hottest jobs:
1. Louisiana( North Western)
Openings for a HistoTech(Bench)
No weekends, No call, and Top Dollar.
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HistoTech Manager
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if you are interested, please call me today at 1-800-466-9919 ext 223
3. Colorado (Greater Denver area)
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13. Indiana
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if you are interested, please call me today at 1-800-466-9919 ext 223
15. Oklahoma
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The clients are currently interviewing candidates - so if you are interested,
please call me today at 1-800-466-9919 ext 223
Thank you,
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1-800-466-9919 ext 223
------------------------------
Message: 15
Date: Wed, 12 Oct 2005 23:06:56 -0700
From: "Bonnie J. McMahill" <bmcmahill <@t> incytepathology.com>
Subject: [Histonet] Height Adjustable tables
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<PATH2K-SRV2v52cpT78000023dc <@t> PATH2K-SRV2.PAI.E-PATHOLOGY.COM>
Content-Type: text/plain; charset="utf-8"
Hi all,
I was wondering if anyone has height adjustable (electronic or crank) tables
for cutting? We are putting some in our lab, but are interested in
purchasing some from a company that has a known track record.
Thanks!
Bonnie McMahill
InCyte Pathology
Spokane, WA
------------------------------
Message: 16
Date: Thu, 13 Oct 2005 11:25:37 +0100
From: "Jonathan Wilson" <wilson_jm <@t> cimar.org>
Subject: [Histonet] SGLT1 antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003101c5cfe0$751b8950$17dea8c0 <@t> ciimar.up.pt>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hello,
Could someone please recommend an antibody against SGLT1 (sodium glucose
transporter) that works in rat or mouse FFPE tissue. I have tried a chemicon
antibody but without any luck.
Thanks for any advice.
Sincerely,
Jonathan Wilson
CIIMAR-Ecofisiologia
Rua dos Bragas 289
4050-123 Porto Portugal
tel 351 22 340 1809
fax 351 22 339 0608
alt. e-mail: mop01258 <@t> mail.telepac.pt
------------------------------
Message: 17
Date: Thu, 13 Oct 2005 12:27:10 +0100
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] RE: beta-2- microglobulin
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC88BEDFD1FC3F468D0376A7C75465F705C7568A <@t> Saffron.cfs.le.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Looking for an antibody to the above that works, as ever, on paraffin
processed mouse tissues...
Many thanks
Richard Edwards
MRC TOX UNIT
LEICESTER...U.K....
------------------------------
Message: 18
Date: Thu, 13 Oct 2005 07:48:24 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] decalcifying solutions for skeletochronology
To: Malcolm McCallum <Malcolm.McCallum <@t> tamut.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051013144824.30771.qmail <@t> web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
The decalcifying solution that you may end using (after any standard recipe
you can find in any good histology manual) will depend on the type of bone
you want to decalcify and what details you want to preserve.
EDTA will give you the most details, but it will take longer; sulfuric acid
will decalcify faster but will destroy almost any detail.
The weaker the acid (lique citric) the slower the process but the more detail
you will preserve; and an inverse correlation you will find with the stronger
the acid.
I hope this will help you to sort out your options.
Rene J.
Malcolm McCallum <Malcolm.McCallum <@t> tamut.edu> wrote:
Hi,
I am having some students do various skeletochronology projects with
amphibians and reptiles. I could just buy some decalcifying solution, but we
have so many acids, I figured I would make some. Any good recipes out there
for decalcifying bone w/o heating?
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843
Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 19
Date: Thu, 13 Oct 2005 08:19:46 -0700 (PDT)
From: Adam Perry <kaleid11 <@t> yahoo.com>
Subject: [Histonet] MOUSE BRAIN FIXATION
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051013151946.35658.qmail <@t> web30411.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I downloaded the technical report on this variant perfusion technique. I'm
amazed that you can get good fixation of the brain with 1 ml of fixative
pumped through the heart without clamping off descending aorta, etc. Has
anyone tried this technique (or similar variants), and what kind of results
were obtained?
I'm planning on trying it in a week or so, because I would love to get good
brain fixation with so little time, fixative and waste production...but am
wary...
Adam Perry
Department of Physiology and Biophysics
University of Illinois
Chicago, IL 60612
There have been several inquiries regarding fixation of mouse brains
recently.
A short technical report has just been published in BioTechniques:
Minimally invasive method for murine brain fixation
Eichenbaum KD et al
Biotechniques 2005; 39(4): 487-500
After simple registration, it can be accessed at
http://www.biotechniques.com <http://www.biotechniques.com>
Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston <@t> bshsi.com
---------------------------------
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------------------------------
Message: 20
Date: Thu, 13 Oct 2005 11:35:32 -0400
From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] MOUSE BRAIN FIXATION
To: 'Adam Perry' <kaleid11 <@t> yahoo.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<A985B7D45D355D4EB363288E717394375B7F98 <@t> NIHCESMLBX5.nih.gov>
Content-Type: text/plain
I have this paper and hope to try this next week. Note that the fixative is
picric acid/paraformaldehyde/gluteraldehyde. I would love to get great
fixation with 1ml and without opening the chest cavity. I will let you know!
c
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
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-----Original Message-----
From: Adam Perry [mailto:kaleid11 <@t> yahoo.com]
Sent: Thursday, October 13, 2005 8:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MOUSE BRAIN FIXATION
I downloaded the technical report on this variant perfusion technique. I'm
amazed that you can get good fixation of the brain with 1 ml of fixative
pumped through the heart without clamping off descending aorta, etc. Has
anyone tried this technique (or similar variants), and what kind of results
were obtained?
I'm planning on trying it in a week or so, because I would love to get good
brain fixation with so little time, fixative and waste production...but am
wary...
Adam Perry
Department of Physiology and Biophysics
University of Illinois
Chicago, IL 60612
There have been several inquiries regarding fixation of mouse brains
recently.
A short technical report has just been published in BioTechniques:
Minimally invasive method for murine brain fixation
Eichenbaum KD et al
Biotechniques 2005; 39(4): 487-500
After simple registration, it can be accessed at
http://www.biotechniques.com <http://www.biotechniques.com>
Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston <@t> bshsi.com
---------------------------------
Yahoo! Music Unlimited - Access over 1 million songs. Try it free.
_______________________________________________
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------------------------------
Message: 21
Date: Thu, 13 Oct 2005 09:32:24 -0700
From: "Arshia Mian" <amian <@t> thermage.com>
Subject: [Histonet] Re: Tissue with Fat
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DB0A3CC79032EF4CAC5E438EF58C5D1E01CDB87D <@t> tmail.thermage.com>
Content-Type: text/plain; charset="us-ascii"
I have been having some problems with cutting Tissue with Fat on a
cryostat (box temp and
object temp are at -20C). The slices I cut tend to curl in the middle
of the block close to the tissue. I have
changed the blade several times, but it still continues to happen even
with a new blade. Is there any correlation
between the box temp and the object temp that would cause this to
happen? Any suggestions?
I have also noticed that at times, even though my setting is set to 20
microns, that I get thick and thin cuts.
Any suggestions on this as well? I would greatly appreciate it. Thanks.
------------------------------
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