[Histonet] tooth structure

[pruegg <@t> ihctech.net]

If you are just trying to maintain anatomy position through out processing,
embedding, sectioning and staining, I use a system with samples where I will
forinstance embed the superior side down with the posterior end pointing
towards the label end of the embedding cassette, when I sectioning I make
sure to take the section off the block without flipping it over and pickup
the section so that the posterior end is pointing towards the label end of
the slide each time.  It takes a little concentration and you have to know
how the tissue came out of the body (I am actually there so that it get
oriented properly) but this works for me.  I hope this helps and is what you
mean.  There are tattoo dyes you can use also to mark tissue.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rittman,
Barry R
Sent: Monday, October 10, 2005 8:53 PM
To: Trisha Emry; histo
Subject: RE: [Histonet] tooth structure

Trisha
Not totally sure what you want.
It would help greatly if you let us know what parameters you wish to measure
concerning the tooth's architecture.
Are you concerned about distortion of the tooth during sectioning and
mounting?
Thanks
Barry
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Trisha Emry
Sent: Mon 10/10/2005 2:11 PM
To: histo
Subject: [Histonet] tooth structure



Hi,

I have been requested to process a "special" tooth.
I need some way to mark the position of the tooth throughout sectioning.

Is there something I can embed and section along with the tooth to mark the
architecture for computer imaging? I am doing this in paraffin.

Thanks,
Trisha
U of WA
Seattle


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