[Histonet] storing fixed cells

[anh2006 <@t> med.cornell.edu]

When I am doing staining as such, I fix in 1-4% PFA for 15 mintues and
then I transfer to PBS (after at least one PBS wash). I often store in PBS
for up to a week (at 4 deg C and parafilmed) before staining with no
obvious change in staining quality.

Andrea

> Hi everyone,
>
> I´m planning to use cells, grown in chamber slides, to
> immunofluorescence. Does anyone out there know if it is possible to
> store these cells (human fibroblasts) for some time after fixation with
> ice-cold  ethanol. Should they be kept in refridgerator or frozen and
> should they be covered by some buffer.
>
> Thanks in advance
>
> Gunilla Aronsson
>
> Göteborg University
> Sweden
>
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