[Histonet] RE: Tissue fixation help

Patsy Ruegg pruegg <@t> ihctech.net
Mon Oct 3 14:45:39 CDT 2005

I too have thawed tissue in rt formalin and then processed and embedded in
paraffin which good morphology and IHC results (haven't tried with
antibodies to phosphorylated sites but other IHC has been fine, it all
depends on if the samples was properly frozen in the first place)  I tried
to do this with some heart tissue which had just been placed in a -80dc
freezer with out snap freezing in OCT and it was awful, but the frozen
section was bad as well.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C.M. van der
Sent: Sunday, October 02, 2005 11:43 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Tissue fixation help


   Long  time  ago  we thawed a  frozen  tissue  sample directly in (room
   temp.)  NBF  (24-48 hs). Morphology was kept surprisingly well. Sorry,
   at that time phosphorylated antibodies were not even existant....

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631

   Date: Fri, 30 Sep 2005 08:25:24 -0400
   From: Angela McNabola <angela.mcnabola.b <@t> bayer.com>
   Subject: [Histonet] Tissue fixation help
   To: [1]Histonet <@t> lists.utsouthwestern.edu
   Good Morning,
   Does  anybody  on  the histonet have any experience with taking tissue
   that has
   been snap frozen in minus 80, and then "thawed" out and formalin fixed
   paraffin embedded?
   I  won't  get into the reasons why we have to do this, but does anyone
   have a
   protocol  (or just slowly thaw it out and fix it??).  How did your IHC
   turn out,
   especially  when  using  phosphorlyated  antibodies?  Did you get good
   morphology of
   your tissue?
   thanks in advance!
   Angela McNabola, MS HT(ASCP)SLS, QIHC
   Bayer Healthcare
   400 Morgan Lane
   West Haven, CT 06516
   angela.mcnabola.b <@t> bayer.com


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