I too have thawed tissue in rt formalin and then processed and embedded in
paraffin which good morphology and IHC results (haven't tried with
antibodies to phosphorylated sites but other IHC has been fine, it all
depends on if the samples was properly frozen in the first place) I tried
to do this with some heart tissue which had just been placed in a -80dc
freezer with out snap freezing in OCT and it was awful, but the frozen
section was bad as well.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C.M. van der
Loos
Sent: Sunday, October 02, 2005 11:43 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Tissue fixation help
Angela,
Long time ago we thawed a frozen tissue sample directly in (room
temp.) NBF (24-48 hs). Morphology was kept surprisingly well. Sorry,
at that time phosphorylated antibodies were not even existant....
Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands
phone: +31 20 5665631
Date: Fri, 30 Sep 2005 08:25:24 -0400
From: Angela McNabola <angela.mcnabola.b <@t> bayer.com>
Subject: [Histonet] Tissue fixation help
To: [1]Histonet <@t> lists.utsouthwestern.edu
Good Morning,
Does anybody on the histonet have any experience with taking tissue
that has
been snap frozen in minus 80, and then "thawed" out and formalin fixed
and
paraffin embedded?
I won't get into the reasons why we have to do this, but does anyone
have a
protocol (or just slowly thaw it out and fix it??). How did your IHC
turn out,
especially when using phosphorlyated antibodies? Did you get good
morphology of
your tissue?
thanks in advance!
Angela
Angela McNabola, MS HT(ASCP)SLS, QIHC
Bayer Healthcare
400 Morgan Lane
West Haven, CT 06516
203-812-5001
angela.mcnabola.b <@t> bayer.com
References
1. mailto:Histonet <@t> lists.utsouthwestern.edu
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