[Histonet] re JKiernan APES treatment of slides

John A. Kiernan jkiernan <@t> uwo.ca
Mon Oct 3 10:06:18 CDT 2005

1. I don't know how commercial positively charged 
slides are made. Try asking the maker. (Good luck!)
It is possible that they use an alkoxysilane other
than APES to achieve a similar effect.
2. Using an acetone rinse to speed up drying should 
not change the APES-treated slides. The aminopropyl
groups are covalently bound to the glass. They
cannot be washed off, and they do not react with acetone.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Carl Hobbs wrote:
>  Thank you very much for your reiteration of the procedure for charging
> > slides, using APES, Mr Kiernan. I would be grateful for your comments
> > regarding:
> > 1. Do you know if  the  "Superfrost plus" slides that I buy from VWR are
> > prepared using the same/similar process?
> > 2. I use a similar method to your  posted one ; it differs only in that I
> > rinse the
> > slides in water after the APES/acetone treatment, then  rinse in acetone
> > to aid a fast dry; do you think that this has any deleterious effect on
> > the charging of the slides?
> > Carl
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