[Histonet] re JKiernan APES treatment of slides

[John A. Kiernan]

1. I don't know how commercial positively charged 
slides are made. Try asking the maker. (Good luck!)
It is possible that they use an alkoxysilane other
than APES to achieve a similar effect.
2. Using an acetone rinse to speed up drying should 
not change the APES-treated slides. The aminopropyl
groups are covalently bound to the glass. They
cannot be washed off, and they do not react with acetone.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Carl Hobbs wrote:
> 
>  Thank you very much for your reiteration of the procedure for charging
> > slides, using APES, Mr Kiernan. I would be grateful for your comments
> > regarding:
> > 1. Do you know if  the  "Superfrost plus" slides that I buy from VWR are
> > prepared using the same/similar process?
> > 2. I use a similar method to your  posted one ; it differs only in that I
> > rinse the
> > slides in water after the APES/acetone treatment, then  rinse in acetone
> > to aid a fast dry; do you think that this has any deleterious effect on
> > the charging of the slides?
> > Carl
> 
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