[Histonet] cryosection bubbles

[Ingles Claire]

We occasionally get bubbles between the section and the slide. It ususally helps if you warm the slide with your thumb, etc, before putting the section on so it adheres faster. I find the slower the section sticks to the slide, the more prevelant air bubbles and wrinkles are. Softly breathing on the slide while the section is adhering helps too.
Claire Ingles
Mohs Clinic, UW Hosp.
Madison WI 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Karl
Sent: Wed 11/30/2005 5:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cryosection bubbles



Dear Histonetters,

When making cryosections of mouse tissues (brain and liver, which is all i
section these days) i get bubble formation between the slide and the
section.

The bubbles are 0.2 -1.0 mm in diameter, and sufficiently large to contact
the blade holder when picking up additional sections on the same slide.

There are no bubbles visible in the OCT, so it seems clear the bubbles are
introduced at the moment the slide is contacting the sections. I get these
bubbles regardless of section thickness (10ums - 25ums).

I'm very satisfied with the sections, but which i could eliminate these
bubbles! Any tips or aspects to pay attention to in this regard are greatly
appreciated.

many thanks in advance,

Karl
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet