[Histonet] NBF or paraformaldehyde

[bhewlett <@t> cogeco.ca]

> Sarah,
There is no difference between 4% NBF and 4% paraformaldehyde in the same buffer! That is because paraformaldehyde in
solution IS formaldehyde.

Bryan
>
> Dear Histonet:
> I should probably know this, but I don't, so I am asking. What is the
> difference between using neutral buffered formalin and paraformaldehyde?
> Does one give better morphology or better recovery of antigens for
> immunostaining? I am a researcher, not a histotech, and I have always
> used para. However, with all this talk of NBF, I am wondering why. I
> believe that I have been told at one time or another that the
> crosslinking is better with para, but since I am always doing
> immunostaining, I don't want strong crosslinking.
>
> I apologize if this is a totally "you should know" question. If there
> are websites describing this, I would appreciate getting them, because
> that will save me some time looking them up.
> Thanks, this is a great listserve!
>
> Sarah Pixley
> Univ. of Cincinnati
>
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