[Histonet] c4d

[Sebree Linda A.]

Hi Gudrun,

For frozen sections we use Biogenesis C4d at 1:600 on our Benchmark XT for 24" with AB block.  We pretreat our acetone fixed sections in VMS Morphosave for 2" but this isn't a critical step and frozens will stain without it.  For paraffin sections, we perform HIER in EDTA in a Biocare decloaking chamber for 3" followed by a 32" incubation and VMS amplification as well as AB block.  We use rejecting kidney as a control as our patient specimens are 95% renal biopsies being tested for possible rejection.

Hope this is of some help,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Tuesday, November 29, 2005 9:18 AM
To: Histonetliste (Histonetliste)
Subject: AW: [Histonet] c4d


Dear Richard,
We are going to introduce this antibody in our lab. We work with the Benchmark XT. Can you give me some hints about the titer and incubation time you use? Or what tissue is preferd as a positiv control? Does it work constantly?

Thank you
Gudrun Lang
Histotech, Akh Linz
Austria


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Richard Cartun
Gesendet: Freitag, 25. November 2005 20:15
An: Tracey.Lenek <@t> CLS.ab.ca; histonet <@t> lists.utsouthwestern.edu
Betreff: Re: [Histonet] c4d

We use a rabbit polyclonal antibody from American Research Products, Inc. located in Belmont, MA.  The catalog number is 12-5000.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> <Tracey.Lenek <@t> CLS.ab.ca> 11/02/05 02:30PM >>>
Could anyone recommend a monoclonal C4d antibody for FFPE human tissue?

Thanks
Tracey

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