[Histonet] Dark staining

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Nov 28 11:12:12 CST 2005


Are the GI biopsies fixed immediately after being taken?
  Do some by any chance air-dry before fixing?
  Are all the sections of the same thickness?
  I would check on these
  Rene J.

"Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:
  I am wondering what causes some of our tissues to stain with hematoxylin
darker than others? The pathologist is noticing it mostly on GI
biopsies or colon biopsies, not all of them on the same day either. I
can't believe it is due to improper fixation, as all of them are getting
practically the same amount of time in formalin and it is very adequate,
often more than 24 hours. Any ideas would be appreciated. We use
Gills I hematoxylin and Eosin from sigma.
________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.

If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  




		
---------------------------------
 Yahoo! Music Unlimited - Access over 1 million songs. Try it free.


More information about the Histonet mailing list