[Histonet] weak antibody signal

Till, Renee TillRenee <@t> uams.edu
Tue Nov 22 10:44:27 CST 2005


We have a BTEB1 goat polyclonal antibody that is staining very weakly
using a Vectastain kit and DAB. I personally like to order my
secondaries and streptavidin separate, that way I can get F(ab')2
fragements or what have you, if I want. I was going to suggest trying
this, as it is has worked better for me on some stains than the Vector
kits. Is there anything else that could be done to boost the signal? I
had thought of CSA kits and the like, but from what I can tell the Dako
kit at least isn't for goat. Would it work better perhaps using
fluorescence instead of DAB? 

 

Renee' Till, HT

 

Arkansas Childrens Nutrition Center

1120 Marshall

Little Rock, AR 

72202

 

(501) 364-2774

 


================================================================================================

Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
================================================================================================


More information about the Histonet mailing list