[Histonet] oil red o

Tue Nov 22 03:21:11 CST 2005

PITA's? Professional International Technical Advisors? Well thank you so
much, it's nice for our talents to be recognised. But I'm still confused
over who is buggering whom (note correct use of grammar).

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Broomall
Sent: 21 November 2005 19:25
To: 'Bryan Llewellyn'; Histonet
Subject: RE: [Histonet] oil red o

You guys are PITA's. (Do I need to spell that one out?) 

Bryan got the meaning exactly. (Good Job!) As in, I hate coverslipping
ORO's
because you can't press on the
slip to get air bubbles out because the ORO will move. It's irritating.

Kristen 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bryan
Llewellyn
Sent: Monday, November 21, 2005 12:07 PM
To: Histonet
Subject: Re: [Histonet] oil red o

The ending "er" in English denotes a person (or thing) who/which does an

action.  To "bug" is a colloquial expression meaning to irritate, so a 
"bugger" (the letter g is doubled to preserve the short vowel sound)
would 
be someone (or something) who irritates someone else.  Is there another 
meaning?

Bryan

----- Original Message ----- 
From: "Rogerson Kemlo (ELHT) Pathology" <Kemlo.Rogerson <@t> elht.nhs.uk>
To: "Kristen Broomall" <kbroomal <@t> NEMOURS.ORG>; "Sally Prouty" 
<prouty27 <@t> msn.com>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, November 21, 2005 8:28 AM
Subject: RE: [Histonet] oil red o

"Quite a bugger" I quote.

Do you know what one is? It doesn't seem to fit the context in which you
are using it. Are you using it as a noun or a verb?

I'm sure you aren't talking about Sally.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristen
Broomall
Sent: Monday, November 21, 2005 4:19 PM
To: 'Sally Prouty'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] oil red o

Make sure that you are not pressing (at all!) on your coverslip, even to
get
out air bubbles. The ORO will come right out of the fat cells. Quite a
bugger.

Kristen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sally
Prouty
Sent: Sunday, November 20, 2005 11:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] oil red o

I am trying to locate a protocol for a consistant Oil red O stain. I
don't
know if it is in coverslipping where the stain is moving off the fat
cells
or in my differentiation step. I am staining on frozen skeletal muscle
sections. Any suggestions would be helpful. Thank you,

Sally

Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944

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