[Histonet] oil red o

[Molinari, Betsy]

Hi Sally,
I use the ORO stain found in Freida Carson's Histotechnology text book
with consistent results. You do have to use a gentle hand when applying
the coverslip and use an aqueous mounting media.

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6524
832-355-6812 (fax)


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sally
Prouty
Sent: Sunday, November 20, 2005 10:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] oil red o

I am trying to locate a protocol for a consistant Oil red O stain. I
don't 
know if it is in coverslipping where the stain is moving off the fat
cells 
or in my differentiation step. I am staining on frozen skeletal muscle 
sections. Any suggestions would be helpful. Thank you,

Sally


Sally J. Prouty
HHMI/University of Iowa
Kevin Campbell Laboratory
400 EMRB
Iowa City, Iowa 52246
319-335-6944

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