[Histonet] best formalin

[Anne Van Binsbergen]

We use Millonigs here too - we make it up in the lab. 
Also used it for many years in my previous lab, always with excellent
results
Annieinarabia

-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu] 
Sent: Wednesday, November 16, 2005 10:10 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] best formalin

We like a neutral buffered formalin (phosphate salts) and purchase it 
either ready to use or in concentrated form (to make a final working 
solution)  We find NBF concentrates convenient and economical. Several
of 
our labs prefer ready to use phophate buffered formalin i.e. 
NBF(Fisherbrand) in order to avoid formaldehyde fumes during NBF 
preparation without an available fume hood.
.
Richard Allan, Surgipath and others have formalin concentrates 
available.  Surgipath has Carsons Millonigs Buffered Formalin with
slightly 
different buffering formulation using sodium hydroxide and a phosphate 
salt, and MBF also comes in concentrated form. The joy of this one is -
it 
was developed for use with both FFPE and EM per Freida Carson's
publication 
in JOH many years ago.

All of the above provided excellent fixation.



Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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