[Histonet] cyrosectioning

[Steven Coakley]

We do allot of cryostat sectioning of both fresh and  fixed sucrose cryoprotected tissue.  I was wondering what other techs have found to result in better sections, one larger piece of tissue or several smaller pieces within one section.  When sectioning several pieces of smaller tissue, such as liver is orientation critical?
 
Thanks everyone.
 
Steve

		
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