[Histonet] Heavy duty bones saw

[Patsy Ruegg]

Years ago some body sold us bone people a manual bone holding and sawing
system, I don't remember the manufacturer, but they still use this at the U
where I retired from.  It was a big metal holding rack with a V shape with
teeth to hold the bone (I used to cut hard to hold round human whole heads
of femur) it used a hack saw/coping saw type saw, there were spaces cut thru
the holder so you could actually cut a pretty thin waffer thru the whole
bone by making two cuts creating a thin waffer between the cuts, it worked
well for getting really large bones down to a more managable size to work
with, the thickness is the most important thing to concern yourself with, if
your bone sample is more than about 3-6 mm it will take forever to
infiltrate.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Monday, November 14, 2005 10:40 AM
To: DiCarlo, Margaret; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Heavy duty bones saw

Some research laboratories use shop band saws and modify them for bone 
work.   The drawback, you need to do something to contain the aerosol 
sprays and bone dust, plus use a device to hold the bone so YOU are not the
specimen.

Fixing the bone a few hours in NBF helps with sawing, you can grip bones
better after a bit of fixation as this seems to make a fatty bones/tissues
less slick.  It is a good idea to hold big bones between two pieces of wood
for griping sample and not damaging the blade.  You need to have 16 tpi or
16 teeth per inch for a very fine cut or you will tear the heck out of the
bone/tissue/marrow cavity.

For really large bones, we also used a hand held hack saw with tungsten
blade,  very crude but works to get a huge bone smaller then use MarMed. 
Still a bit messy but containable.  Check out some of the water cooled
Buehler units, but these are very pricey.

We had a huge floor standing meat cutters saw (Hobart) that we could water
cool but this is a monster, and was located in a separate room with a drain
in the floor.

At 08:52 AM 11/11/2005, you wrote:
>I would also be interested in a heavy duty band saw since I work with 
>large human bones.  When I looked into the Mar-Med I felt it was not 
>sturdy or large enough to meet my lab needs.
>
>I also would appreciate any suggestions.
>
>Peggy DiCarlo HT (ASCP)
>Orthopedics Bone Lab
>Buffalo General Hospital
>100 High St.
>Buffalo, NY  14203
>716-859-1293
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Temple 
>Nancy
>Sent: Friday, November 11, 2005 08:47
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Bone Saw
>
>
>We are currently using a bone saw made by Mar-Med. We do a lot of 
>bones, and are in need of a heavier duty bone saw. We have had to 
>replace the motor twice this year on the Mar-Med. Any suggestions?
>Thanks,
>
>Nancy
>St. Francis Hospital
>Indianapolis, In
>
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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