[Histonet] biotin blocking for frozen sections

[Gayle Callis]

We have used Vectors Avidin/biotin kits.  Now, however, with our 
Strepavidin- Biotin methods, we now use their Strepavidin/biotin blocking kit.

I have heard that DAKO's avidin/biotin blocking kit is stronger, more 
concentrated in order to block endogenous and unwanted biotins when using 
the CSA or tyramide amplification method (TSA) - certainly worth a try.



At 09:10 AM 11/14/2005, you wrote:
>For FROZEN sections of mouse tissue, what are people using to block 
>biotin. I am having problems with the standard kits I use.
>
>Thanks,
>Andrea
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)