[Histonet] IgG detection for mouse tissue?
Gayle Callis
gcallis <@t> montana.edu
Mon Nov 14 11:49:56 CST 2005
Yes, but to do this, we prefer to use a monoclonal (BD Pharmingen) that is
Rat antiMouse IgG. If you use a polyclonal, go to Southern Biotechnology
and make sure the antibody is adsorbed to mouse tissue, and/or an F(ab')2
frag of IgG to avoid fc receptor problems.
OR use an antiAt 05:59 PM 11/11/2005, you wrote:
>Hi All,
> Does any one know whether there are antibodies to detect mouse IgG on
> mouse tissues? Thank you for the help.
>
>
>
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
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