[Histonet] any suggestions?

[Pamela Marcum]

Thank You Gayle!!  It is sometimes easy to forget not everyone has access 
to the Internet or a way to even place a message on HistoNet.  We are 
attempting to assist people who are learning and while some may seem to be 
using HistoNet as way to avoid looking anything up the majority are asking 
questions that are not readily accessible or in an area  like murine animal 
models that is more difficult.  I have no problem with this and will answer 
most questions off line if it is a more esoteric  question.  I am surprised 
that the person with the issue about Rene's assistance for her friend is 
also the same Rene who has been asking all of us to contribute to Tricks of 
the Trade.

Pamela A Marcum
Special Procedures Manager
Bone Histology
UPENN Vet School


At 05:33 PM 11/9/2005, Gayle Callis wrote:
>Rene,
>
>Sorry to disagree, and I certainly did not understand what YOU meant by 
>"changing his ways"?  I couldn't see many changes here, other than some 
>refinement of his IHC method.  Many people working with murine CD marker 
>IHC often need a little help with murine  animal model IHC.   Since our 
>lab works with murine CD marker IHC approx. 95% of the time, on frozen 
>sections etc per the inquiry, we are happy to sleuth problems.  The 
>questions were valid ones for learning/refining of his techniques and 
>being a mousie person, I didn't have a problem with the inquiry.
>
>Some people (including a lot of experts in our field) do NOT want to be on 
>Histonet and second hand messaging certainly doesn't bother me as long as 
>more details are provided.  I frequently ask questions on Histonet to 
>access information for others in my department - that's part of my job. 
>Your colleague is welcome to email privately if you or they wish.
>
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
>
>
>
>- At 02:28 PM 11/9/2005, you wrote:
>>With all due respect I think that your colleague's procedure is a royal mess.
>>On the other hand it seems that he is not very willing to change his 
>>ways, so the only thing I have to tell you (for him) is: good luck! He 
>>should be the one doing the asking, and not you!
>>Rene J.
>
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
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