[Histonet] Histologist ratio
Clarke, Mary
mclarke <@t> allsaintshealthcare.org
Tue Nov 8 11:05:52 CST 2005
We have 4 pathologists and 4 histotechs, one who is registry eligible and 3 who are registered.
Terri Clarke
Histology Supervisor
All Saints Laboratory
3801 Spring Street
Racine, Wi 53405
mclarke <@t> allsaintshealthcare.org
262-687-6609
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, November 08, 2005 9:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 24, Issue 10
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. RE: measuring collagen (Boyce, Amanda (NIH/NIAMS))
2. H. Pylori Controls (Vicki Gauch)
3. Re: H. Pylori Controls (Rene J Buesa)
4. histologist/pathologist ratio (LINDA MARGRAF)
5. RE: H. Pylori Controls (Jeff Gordon)
6. Sheep anti BrdU (Yun Li)
7. Re: histologist/pathologist ratio (Rene J Buesa)
8. Sihler's Stain (Dianne Holmes)
9. (no subject) (mplhisto <@t> aol.com)
10. Re: Sihler's Stain (Rene J Buesa)
11. Inflammation (John PJ Coleman)
12. Histo Online Program (Linresearch <@t> aol.com)
13. Pleaes unsuscribe (Bernardo Vargas Angel)
14. re: grinding bone sections (caron fournier)
15. cytospin or cytek machine (Elizabeth Chlipala)
16. Re: Sihler's Stain (John Kiernan)
17. Re: re: grinding bone sections (louise renton)
18. Re: Bone marrow unspecific staining (Mikael Niku)
19. Thermal polymerisation of LR gold resin for electron
microscopy (Peter Bannister)
20. Red marker (Steven Coakley)
21. Mounting Gelatine Embedded Cryostat CNS Sections
(germckeon <@t> excite.com)
22. RE: Red marker (Ingles Claire)
23. Reagent Grade Alcohol (LeVier, Rebecca J)
24. Re: Bone marrow unspecific staining (Rene J Buesa)
25. RE: histologist/pathologist ratio (Horn, Hazel V)
26. Re: Reagent Grade Alcohol (Rene J Buesa)
27. Thank you (Vicki Gauch)
28. RE: histologist/pathologist ratio (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Mon, 7 Nov 2005 13:18:26 -0500
From: "Boyce, Amanda \(NIH/NIAMS\)" <boycea <@t> mail.nih.gov>
Subject: [Histonet] RE: measuring collagen
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0E3E7E8F6E23DF4C8127A063568356B510B98802 <@t> nihexchange12.nih.gov>
Content-Type: text/plain; charset="iso-8859-1"
Our lab uses a hydroxyproline assay. I've never used it, so I can't troubleshoot it for you, but here's the protocol:
Acid hydrolysis
* Papain digest and 12N HCL 1:1
* 110 overnight in sealed glass ampules
* dry sample 24-48 h in vacuum-pump with NaOH
* resuspend in 1 ml assay buffer, vortex, overnight at 4, vortex again
* store at -20 or assay
Hydroxyproline assay
Reagents:
* stock buffer: 50 g citric acid monohydrate, 12 ml glacial acetic acid , 120 g sodium acetate trihydrate, 34 g sodium hydroxide ad 1 liter, pH 6.0, store at 4
* assay buffer: 1:10 dilution of stock buffer
* chloramin-T-reagent: 0.3525g Chloramine T dissolved in 5.175 ml water, add 6.5 ml m-propanol and 13.325 ml stock buffer, make fresh
* Dimethylaminobenzaldehyde reagent: 3.75 g dimethylaminobenzaldehyde in 15 ml n-propanol, add 6.5 ml perchloric acid, make fresh
* Standard: 1 mg/ml hydroyxproline, store -20
Method:
1. standard dilutions: 0, 0 , 0.2, 0.4, 0.6, 0.8, 1, 2, 4, 6, 8 ,10 µg/ml
2. sample dilutions: 1:5, 1:10, 1:20, 1:40, 1:80,1: 100
3. 200 µl sample/standard in eppendorf
4. add 100 µl Chloramine T, pipett mix 20 min room temp
5. add 100 µl dimethylaminobenzaldehyde, pipet mix, 15 min 60 water bath
6. 300 µl in 96 well plate and read immediately 540 nm
--------------------------
Amanda Taylor Boyce, Ph.D.
Postdoctoral IRTA Fellow
Cartilage Biology and Orthopaedics Branch
National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institutes of Health Bldg. 13, Rm. 3W17 Bethesda, MD 20892-5755
Phone: 301-451-6860
Fax: 301-480-4315
Email: boycea <@t> mail.nih.gov
> Message: 6
> Date: Mon, 7 Nov 2005 07:00:09 -0800 (PST)
> From: Niloufar Fozouni <niloof <@t> yahoo.com>
> Subject: [Histonet] measuring collagen
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20051107150019.64420.qmail <@t> web35602.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hello,
>
> Does anybody know, how I can measure collagen content
> in cartilage?
> Thank you.
> Niloo
>
>
------------------------------
Message: 2
Date: Mon, 07 Nov 2005 13:24:25 -0500
From: "Vicki Gauch" <GauchV <@t> mail.amc.edu>
Subject: [Histonet] H. Pylori Controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s36f55ad.067 <@t> amc03.amc.edu>
Content-Type: text/plain; charset="US-ASCII"
Does anyone know of a good source for H. Pylori Controls?? We normally order from Newcomer Supply but they are experiencing a large backorder right now and we need something to hold us over until they arrive. Any help would be greatly appreciated... Thanks so much, Vicki Gauch AMCH Albany, NY
-----------------------------------------
CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu.
------------------------------
Message: 3
Date: Mon, 7 Nov 2005 11:30:08 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] H. Pylori Controls
To: Vicki Gauch <GauchV <@t> mail.amc.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051107193008.74020.qmail <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We used positive cases that were selected by the pathologists, but there is another approarch. Usually pathologists want to be able to have a positive control of exactly what they are looking for; if this is the case with your pathologists you will need controls comntaining H. pylori. Now, the whole idea with the control is to make sure that the procedure worked, and this you can obtain with any tissue containing abundant number of bacteria. After a long "convincing" process with our pathologists I was able to make them aware of this fact. We used the Steiner method (with phosphotungstic acid instead of the radiactive uranyl nitrate) and for control we used appendix (and you will not find a more easy to find tissue with more bacteria than an appendix). Perhaps you would like to try to talk about this approach with your pathologist. Just a thought! Rene J.
Vicki Gauch <GauchV <@t> mail.amc.edu> wrote:
Does anyone know of a good source for H. Pylori Controls?? We normally order from Newcomer Supply but they are experiencing a large backorder right now and we need something to hold us over until they arrive. Any help would be greatly appreciated... Thanks so much, Vicki Gauch AMCH Albany, NY
-----------------------------------------
CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
Message: 4
Date: Mon, 07 Nov 2005 13:49:23 -0600
From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
Subject: [Histonet] histologist/pathologist ratio
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s36f5b74.090 <@t> mail.childrens.com>
Content-Type: text/plain; charset=US-ASCII
Here's a message Beatrice is having trouble posting to the list.
Please email her or send your reply to the entire list (and not me directly). Thanks Linda M (Histonet administrator)
"DeBrosse_Beatrice" <DeBrosse_Beatrice <@t> Allergan.com>
Hi!
I would like to know what the ratio of how many histologists to a Pathologist is. Does the ratio differ from hospitals to reference labs to pharmaceutical labs? Your help is greatly appreciated.
Sincerely,
Beatrice DeBrosse-Serra
HT(ASCP)QIHC
Allergan, Inc.
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5116
------------------------------
Message: 5
Date: Mon, 7 Nov 2005 14:16:27 -0600
From: "Jeff Gordon" <JGordon <@t> cellmarque.com>
Subject: RE: [Histonet] H. Pylori Controls
To: "Vicki Gauch" <GauchV <@t> mail.amc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AE107C473C4B954E80A12057A86FCA836C3951 <@t> cmmail.cellmarque.local>
Content-Type: text/plain; charset="utf-8"
Cell Marque offers H. pylori positive controls for IHC, as well as controls for every other antibody that we carry.
Jeff Gordon
Cell Marque Corp.
http://www.cellmarque.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Vicki Gauch
Sent: Mon 11/7/2005 12:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc:
Subject: [Histonet] H. Pylori Controls
Does anyone know of a good source for H. Pylori Controls?? We normally
order from Newcomer Supply but they are experiencing a large backorder
right now and we need something to hold us over until they arrive.
Any help would be greatly appreciated...
Thanks so much,
Vicki Gauch
AMCH
Albany, NY
-----------------------------------------
CONFIDENTIALITY NOTICE: This email and any attachments may contain
confidential information that is protected by law and is for the sole
use of the individuals or entities to which it is addressed. If you are
not the intended recipient, please notify the sender by replying to
this email and destroying all copies of the communication and
attachments. Further use, disclosure, copying, distribution of, or
reliance upon the contents of this email and attachments is strictly
prohibited. To contact Albany Medical Center, or for a copy of our
privacy practices, please visit us on the Internet at www.amc.edu.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 07 Nov 2005 14:21:17 -0600
From: Yun Li <yun.li <@t> utsouthwestern.edu>
Subject: [Histonet] Sheep anti BrdU
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <436FB73D.3090606 <@t> utsouthwestern.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Greetings everyone,
I am trying to do some double immuno-staining for BrdU and other
antigens. Since the antibodies for my "other" antigens are mostly from
mouse, I am looking for a non-mouse (and preferably non-rat) antibody
against BrdU. I came across this sheep anti brdU antibody in the
literature, it seems that several different companies offer it,
including maine biotech, abcam, novus and one called genetex. So my
question is has anyone here used it before, what type of sections was
used, how did it work and which company was it purchased from? Thanks a
lot!
yun
------------------------------
Message: 7
Date: Mon, 7 Nov 2005 12:43:36 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] histologist/pathologist ratio
To: LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051107204336.58622.qmail <@t> web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Beatrice:
Some months ago I reviewed different sources (US Dept Labor; US Dept. Commerce;ASCP) and got to the figure of about 1.4 to 2.0 histotechs per pathologist. This, as any other average, is "not written in stone" and has to vary between laboratories. Of the laboratories I personally know the averages have been from 1 to 1, to 2.2 to 1 These figures will also depend on the type of laboratory: laboratories in small hospitals will probably will be closer to 1:1, in large reference laboratories, although will have many more pathologists, the huge sample volume will require the larger side of the scale (2 or 2:2 histotechs / pathologist). Rene J.
LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com> wrote:
Here's a message Beatrice is having trouble posting to the list.
Please email her or send your reply to the entire list (and not me directly). Thanks Linda M (Histonet administrator)
"DeBrosse_Beatrice"
Hi!
I would like to know what the ratio of how many histologists to a Pathologist is. Does the ratio differ from hospitals to reference labs to pharmaceutical labs? Your help is greatly appreciated.
Sincerely,
Beatrice DeBrosse-Serra
HT(ASCP)QIHC
Allergan, Inc.
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5116
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
Message: 8
Date: Mon, 07 Nov 2005 15:34:24 -0600
From: "Dianne Holmes" <dholmes <@t> anatomy.umsmed.edu>
Subject: [Histonet] Sihler's Stain
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s36f740f.025 <@t> GWIA1.umsmed.edu>
Content-Type: text/plain; charset=US-ASCII
Is anyone familiar with this process to counterstain nerve fibers within a relatively transparent muscle? I need a materials and method list. Can anyone help me?
------------------------------
Message: 9
Date: Mon, 07 Nov 2005 16:56:31 -0500
From: mplhisto <@t> aol.com
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C7B1FD3F626F95-B64-AA33 <@t> FWM-R21.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Can anyone tell me the name of a good computer safety training program ? we are looking to update our current program ? Thanks
Meredith Hale HT (ASCP)
Histology Supervisor
Pathology Partners
Irving, Texas
------------------------------
Message: 10
Date: Mon, 7 Nov 2005 14:03:50 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Sihler's Stain
To: Dianne Holmes <dholmes <@t> anatomy.umsmed.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051107220350.18981.qmail <@t> web61225.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Dianne:
Sihler's method was published by Bohm and Oppel in 1907 and is used in pieces of fesh muscle. The solutions are:
A- water 75 mL + glycerol 12 mL+ acetic acid 12 mL + chloral hydrate 0.75 g
B- water 75 ml + Ehrlich hematoxylin (1886 formula) 12 mL + glycerol 12 mL + chloral hydrate 0.75 g
C- 0.1% acetic acid in glycerol.
Method: pieces of fresh muscle in Sol.A for 18 hours; transfer to Sol.B for 3 to 10 days; transfer to Sol.C untill differentiated, mount in glycerol, seal coverslip with wax. Hope this will help! Rene J.
Dianne Holmes <dholmes <@t> anatomy.umsmed.edu> wrote:
Is anyone familiar with this process to counterstain nerve fibers within a relatively transparent muscle? I need a materials and method list. Can anyone help me?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
Message: 11
Date: Mon, 7 Nov 2005 17:23:10 -0500
From: John PJ Coleman <jcolclefa <@t> aol.com>
Subject: [Histonet] Inflammation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <ca067070c8ac9a505bd8df598610d533 <@t> aol.com>
Content-Type: text/plain; charset=US-ASCII; format=flowed
For inflammation (?) I personally would start trying to label the
leucocytes. What about CD15 for Polys, CD117 for mast cells, and CD30
or CD68 for Histiocytes?
On Nov 7, 2005, at 1:01 PM, histonet-request <@t> lists.utsouthwestern.edu
wrote:
Dear All,
I wish to use IHC for the detection of chronic inflammation in
human
tissues - specifically within the prostate. I am using formalin
fixed
sections of prostate. The list of possible markers for
chronic
inflammation out there is rather overwhelming. I was wondering
if
anyone had a small panel of tried and tested antibodies that
would
cover most of the chronic inflammatory cells or give a
good
representative picture of chronic inflammation within the tissue.
Or
even suggest a few antibodies with which I could start with.
Many thanks for attention,
Steve Leung
Clinical Research Fellow
University of Edinburgh
U.K.
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 24, Issue 9
***************************************
JPJC-757 335-2159 http://journals.aol.com/jcolclefa/Waytoomuchboringcraptosayusually/
------------------------------
Message: 12
Date: Mon, 7 Nov 2005 17:24:57 EST
From: Linresearch <@t> aol.com
Subject: [Histonet] Histo Online Program
To: histonet <@t> pathology.swmed.edu
Message-ID: <252.72dde0.30a12e39 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello,
Does anyone know of a current online program for Histology?
Lin
------------------------------
Message: 13
Date: Mon, 7 Nov 2005 18:17:21 -0500
From: Bernardo Vargas Angel <vargasb <@t> nova.edu>
Subject: [Histonet] Pleaes unsuscribe
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <1131405441.436fe08192c3b <@t> mail2.nova.edu>
Content-Type: text/plain
Please unsuscribe
Bernardo Vargas Angel Ph.D
------------------------------
Message: 14
Date: Mon, 7 Nov 2005 18:54:26 -0500 (EST)
From: caron fournier <caron_fournier <@t> yahoo.ca>
Subject: [Histonet] re: grinding bone sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051107235426.70464.qmail <@t> web36313.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All:
what is the finest paper that you have used on an exakt system for grinding bone samples (undecalcified). We were told that the 2500 was the highest they had but there is literature that notes 4000.
Caron Fournier, BSc, R.T.
Department of Orthopaedics,
Division of Orthopaedic Engineering Research,
U.B.C.
---------------------------------
Find your next car at Yahoo! Canada Autos
------------------------------
Message: 15
Date: Mon, 7 Nov 2005 17:36:36 -0700
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] cytospin or cytek machine
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <000001c5e3fc$79e12870$a7d48a80 <@t> AMY>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters
I have a unique request. I have a client that is in Emeryville California and they need access to a cytospin or a Thin Prep machine, they are willing to pay for time, etc. If there is anyone out there that is willing to do this can they e-mail me back with contact information so I can forward it to them.
Thanks in advance
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
------------------------------
Message: 16
Date: Tue, 08 Nov 2005 00:15:03 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Sihler's Stain
To: Rene J Buesa <rjbuesa <@t> yahoo.com>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <43703457.F66DB4E3 <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii
Before going ahead with Sihler's haemalum method (for peripheral nerves in cleared whole mounts) consult recent publications:
Wu BL, Sanders I (1994) The Sihler stain: A technique for studying peripheral nerves in whole-mount specimens. Neuroscience Protocols Module 4: 83-93.
Wu B, Saunders I (1994) The Sihler stain: a technique for studying peripheral nerves in whole-mount specimens. In: FG Wouterlood, Neuroscience Protocols. Elsevier, Amsterdam. pp. 94-050-07-01 to 94-050-07-10.
Mu LC, Sanders I (1998) Neuromuscular organization of the human upper esophageal sphincter. Annals of Otology Rhinology and Laryngology 107: 370-377.
Mu LC, Sanders I (1999) Neuromuscular organization of the canine tongue. Anatomical Record 256: 412-424.
Gozil R, Kadioglu D, Calguner E, Erdogan D, Bahcelioglu M, Elmas C (2002) Branching patterns of rabbit oculomotor and trochlear nerves demonstrated by Sihler's stain technique. Biotechnic & Histochemistry 77: 21-25.
This is an important method for examining the "gross" anatomy of small nerves.
Rene: do you have a copy of Bohm & Appel 1907 or of any of Sihler's papers? If so, I'd be really grateful for bibliographic details!
John Kiernan
Anatomy, UWO
London, Canada. ______________________________________________________________
Rene J Buesa wrote:
>
> Hi Dianne:
> Sihler's method was published by Bohm and Oppel in 1907 and is used in
> pieces of fesh muscle. The solutions are:
> A- water 75 mL + glycerol 12 mL+ acetic acid 12 mL + chloral hydrate 0.75 g
> B- water 75 ml + Ehrlich hematoxylin (1886 formula) 12 mL + glycerol 12 mL + chloral hydrate 0.75 g
> C- 0.1% acetic acid in glycerol.
> Method: pieces of fresh muscle in Sol.A for 18 hours; transfer to Sol.B for 3 to 10 days; transfer to Sol.C untill differentiated, mount in glycerol, seal coverslip with wax.
> Hope this will help!
> Rene J.
>
> Dianne Holmes <dholmes <@t> anatomy.umsmed.edu> wrote:
> Is anyone familiar with this process to counterstain nerve fibers
> within a relatively transparent muscle? I need a materials and method
> list. Can anyone help me?
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ---------------------------------
> Yahoo! FareChase - Search multiple travel sites in one click.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Tue, 8 Nov 2005 08:55:14 +0200
From: louise renton <louise.renton <@t> gmail.com>
Subject: Re: [Histonet] re: grinding bone sections
To: caron fournier <caron_fournier <@t> yahoo.ca>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<e483362e0511072255o19c1561ey20e22a82e1140d27 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Yep,
we GRIND on 2500, but POLISH on 4000. This help??
On 11/8/05, caron fournier <caron_fournier <@t> yahoo.ca> wrote:
> Hi All:
> what is the finest paper that you have used on an exakt system for
> grinding bone samples (undecalcified). We were told that the 2500 was
> the highest they had but there is literature that notes 4000.
>
>
> Caron Fournier, BSc, R.T.
> Department of Orthopaedics,
> Division of Orthopaedic Engineering Research,
> U.B.C.
>
>
>
> ---------------------------------
> Find your next car at Yahoo! Canada Autos
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"....onwards through the fog!"
------------------------------
Message: 18
Date: Tue, 08 Nov 2005 10:08:19 +0200
From: Mikael Niku <mikael.niku <@t> helsinki.fi>
Subject: Re: [Histonet] Bone marrow unspecific staining
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <43705CF3.4010806 <@t> helsinki.fi>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Andrea and Rene, thanks for your comments. I'm sorry for being a bit
unspecific with my unspecificity question :)
Some more details on the problem:
- Seems to be caused by unspecific antibody binding, but not a specific
property of this primary ab
- Primary antibody works very well with other tissues
- Also other primaries (and secondary alone) show unspecific staining
with BM
- Not caused by endogenous biotin, peroxidase, or autofluorescence (done
enough controls)
And some more details on the protocol:
- Bovine BM tissue samples decalcified with EDTA, or cell samples
embedded in agar
- Paraformaldehyde fixation
- HIER (glycine-HCl pH 3) + Tween-20 permeabilization + mild protease
digestion
- For peroxidase-based protocol, biotin block (tried also peroxidase
block, doesn't help)
- Serum block
- Rabbit primary antibody (overnight +4C)
- Sheep secondary antibody (biotinylated or fluorescent)
- For biotinylated secondary, tyramide amplification & DAB reaction,
hematoxylin counterstain
- For fluorescent secondary, Sudan Black B for autofluorescence suppression
- Embedding with Faramount
--
////////////////////////////////////////////////////////////
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mitäkö mieltä olen länsimaisesta sivistyksestä?
Minusta se olisi erinomainen ajatus!
- Gandhi
////////////////////////////////////////////////////////////
------------------------------
Message: 19
Date: Tue, 08 Nov 2005 12:07:52 +0000
From: "Peter Bannister" <peter_bannister <@t> hotmail.co.uk>
Subject: [Histonet] Thermal polymerisation of LR gold resin for
electron microscopy
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY113-F4FC0DE0F6D9519AD8D503C1640 <@t> phx.gbl>
Content-Type: text/plain; format=flowed
Hello Histonetters,
Does anyone have a method for the thermal polymerisation of LR gold resin
for electron microscopy?
I am trying to produce blocks of cultured cell pellets fixed in para/glut,
post fixed in Osmium tetroxide, dehydrated in ethanol, infiltrated with the
resin and cured.
I have tried polymerisation using light with mixed results - non osmium
treated blocks polymerise okay and are just about suitable for sectioning.
However, osmium treated blocks will only polymerise down as far as the cell
pellet (I understand that the dark colour absorbs light and therefore
interferes with the polymerisation). I have tried to keep exposure to oxygen
during polymerisation to a minimum by using closed BEEM capsules or snap fit
gelatin capsules. I would therefore like to try the more classical thermal
polymerisation at +60°C.
The problem is that when I add the initiator (benzoyl peroxide) to the pure
resin I end up with a solid mass of resin even before the peroxide is fully
dissolved, at room temperature -so i can't use it on my samples. If anyone can help me out with a protocol that will ensure the resin only
polymerises quickly when at heated to +60°C it would be very much
appreciated.
Many thanks for your time,
Peter Bannister.
Dr. Peter Bannister,
Thrombosis Research Institute,
Histopathology,
Emmanuel Kaye Building,
Manresa Road,
London SW3 6LR. UK.
_________________________________________________________________
Be the first to hear what's new at MSN - sign up to our free newsletters!
http://www.msn.co.uk/newsletters
------------------------------
Message: 20
Date: Tue, 8 Nov 2005 05:47:03 -0800 (PST)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] Red marker
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051108134703.44353.qmail <@t> web90207.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Does anyone know of a company that makes permanant red markers that resist organic solvents?
Thanks,
Steve
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
Message: 21
Date: Tue, 8 Nov 2005 08:51:01 -0500 (EST)
From: "germckeon <@t> excite.com" <germckeon <@t> excite.com>
Subject: [Histonet] Mounting Gelatine Embedded Cryostat CNS Sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051108135101.79C1B3E01 <@t> xprdmailfe6.nwk.excite.com>
Content-Type: text/plain; charset="us-ascii"
Hello,
I am currently working on spinal cord and am having trouble with sections floating off. I have embedded with gelatine, am performing cryostat sectioning and will be doing some of the more structural stains eg Kultschitsky's stain for myelin. I have been using Superfrost slides.
Can anyone advise me regarding choices of slides, coatings, methodologies, etc which would maximise adherence?
In gratitude for any advice received,
Gerald McKeon
_______________________________________________
Join Excite! - http://www.excite.com
The most personalized portal on the Web!
------------------------------
Message: 22
Date: Tue, 8 Nov 2005 08:00:50 -0600
From: "Ingles Claire" <c.ingles <@t> hosp.wisc.edu>
Subject: RE: [Histonet] Red marker
To: "Steven Coakley" <sjchtascp <@t> yahoo.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<583D3E9A1E843445BD54E461D1A2F6F3025ACE6D <@t> uwhis-xchng2.hosp.wisc.edu>
Content-Type: text/plain; charset="utf-8"
I believe I just saw some new ones in a Market lab catalogue yesterday. 1-800-237-3604 or www.marketlabinc.com. :)
Claire Ingles
UW Mohs Clinic Lab
Madison Wisconsin
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Steven Coakley
Sent: Tue 11/8/2005 7:47 AM
To: Histonet <@t> lists.utsouthwestern.edu
Cc:
Subject: [Histonet] Red marker
Does anyone know of a company that makes permanant red markers that resist organic solvents?
Thanks,
Steve
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 23
Date: Tue, 8 Nov 2005 09:38:41 -0500
From: "LeVier, Rebecca J" <RJLevier <@t> LancasterGeneral.org>
Subject: [Histonet] Reagent Grade Alcohol
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D25E2214 <@t> MAIL-LR.lha.org>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters,
I was just wondering what everyone was using for alcohol. Does anyone see any differences in Reagent Grade Alcohol compared to LCB regulated alcohol? ...... Any information on this topic would be greatly appreciated.
Thanks in advance.
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use of intended recipient(s) and may
contain confidential and privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not
the intended recipient, please contact the sender by reply e-mail and
destroy all copies of the original message.
------------------------------
Message: 24
Date: Tue, 8 Nov 2005 06:50:28 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Bone marrow unspecific staining
To: Mikael Niku <mikael.niku <@t> helsinki.fi>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051108145028.81939.qmail <@t> web61218.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Although I cannot be absolutely sure, for me it seems that perhaps the problem may reside in the paraformaldehyde fixation. Do you have to use it? Could you use another fixative in another parallel sample just to try to find out? Just a thoght! Rene J.
Mikael Niku <mikael.niku <@t> helsinki.fi> wrote:
Andrea and Rene, thanks for your comments. I'm sorry for being a bit
unspecific with my unspecificity question :)
Some more details on the problem:
- Seems to be caused by unspecific antibody binding, but not a specific
property of this primary ab
- Primary antibody works very well with other tissues
- Also other primaries (and secondary alone) show unspecific staining
with BM
- Not caused by endogenous biotin, peroxidase, or autofluorescence (done
enough controls)
And some more details on the protocol:
- Bovine BM tissue samples decalcified with EDTA, or cell samples
embedded in agar
- Paraformaldehyde fixation
- HIER (glycine-HCl pH 3) + Tween-20 permeabilization + mild protease
digestion
- For peroxidase-based protocol, biotin block (tried also peroxidase
block, doesn't help)
- Serum block
- Rabbit primary antibody (overnight +4C)
- Sheep secondary antibody (biotinylated or fluorescent)
- For biotinylated secondary, tyramide amplification & DAB reaction,
hematoxylin counterstain
- For fluorescent secondary, Sudan Black B for autofluorescence suppression
- Embedding with Faramount
--
////////////////////////////////////////////////////////////
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mitäkö mieltä olen länsimaisesta sivistyksestä?
Minusta se olisi erinomainen ajatus!
- Gandhi
////////////////////////////////////////////////////////////
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
Message: 25
Date: Tue, 8 Nov 2005 08:56:38 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] histologist/pathologist ratio
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "LINDA MARGRAF"
<LINDA.MARGRAF <@t> childrens.com>, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C63038BDE9A <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii
We have 5 anatomic pathologists and 3 histotechs.
Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital
800 Marshall Mail Slot 820
Little Rock, AR 72202
phone- 501.364.4240
fax- 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, November 07, 2005 2:44 PM
To: LINDA MARGRAF; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] histologist/pathologist ratio
Hi Beatrice:
Some months ago I reviewed different sources (US Dept Labor; US Dept.
Commerce;ASCP) and got to the figure of about 1.4 to 2.0 histotechs per pathologist. This, as any other average, is "not written in stone" and has to vary between laboratories. Of the laboratories I personally know the averages have been from 1 to 1, to 2.2 to 1 These figures will also depend on the type of laboratory: laboratories in small hospitals will probably will be closer to 1:1, in large reference laboratories, although will have many more pathologists, the huge sample volume will require the larger side of the scale (2 or 2:2 histotechs / pathologist). Rene J.
LINDA MARGRAF <LINDA.MARGRAF <@t> childrens.com> wrote:
Here's a message Beatrice is having trouble posting to the list.
Please email her or send your reply to the entire list (and not me directly). Thanks Linda M (Histonet administrator)
"DeBrosse_Beatrice"
Hi!
I would like to know what the ratio of how many histologists to a Pathologist is. Does the ratio differ from hospitals to reference labs to pharmaceutical labs? Your help is greatly appreciated.
Sincerely,
Beatrice DeBrosse-Serra
HT(ASCP)QIHC
Allergan, Inc.
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5116
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------------------------------------------------------
The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you.
==============================================================================
------------------------------
Message: 26
Date: Tue, 8 Nov 2005 06:59:22 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Reagent Grade Alcohol
To: "LeVier, Rebecca J" <RJLevier <@t> LancasterGeneral.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051108145923.84304.qmail <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I always used 100% (= "200 grade") ,bought in bulk tax exempted, ethanol. Anything you need to do with "pure" ethanol will be simpler to calculate (solutions, mixtures, etc.) with 100% ethanol. Besides that, the "denatured" ethanol contains substances other than ethanol. Now, "reagent grade" sold by chemical companies with "impurities certificates", are much more expensive and offer no advantage over the type of "200 grade" ethanol I used to use. Rene J.
"LeVier, Rebecca J" <RJLevier <@t> LancasterGeneral.org> wrote: Hello Histonetters,
I was just wondering what everyone was using for alcohol. Does anyone see any differences in Reagent Grade Alcohol compared to LCB regulated alcohol? ...... Any information on this topic would be greatly appreciated.
Thanks in advance.
Confidentiality Notice: This e-mail message, including any
attachments, is for the sole use of intended recipient(s) and may
contain confidential and privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not
the intended recipient, please contact the sender by reply e-mail and
destroy all copies of the original message. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
Message: 27
Date: Tue, 08 Nov 2005 10:00:22 -0500
From: "Vicki Gauch" <GauchV <@t> mail.amc.edu>
Subject: [Histonet] Thank you
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s3707750.013 <@t> amc03.amc.edu>
Content-Type: text/plain; charset="US-ASCII"
Thank you to everyone who responded to my query for H. Pylori Controls...we are looking into them now....You really are GREAT !!! Vicki AMCH Albany
-----------------------------------------
CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu.
------------------------------
Message: 28
Date: Tue, 8 Nov 2005 07:02:52 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: RE: [Histonet] histologist/pathologist ratio
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>, LINDA MARGRAF
<LINDA.MARGRAF <@t> childrens.com>, Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20051108150252.25624.qmail <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Question to all subscribers: why don't you post the histologists/pathologists ratios you have in each of your own laboratories? It will be like a survey from Histonet that for sure will usefull for all. Yesterday I posted the ratois I was aware of; now is your turns! Rene J.
"Horn, Hazel V" <HornHV <@t> archildrens.org> wrote:
We have 5 anatomic pathologists and 3 histotechs.
Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital
800 Marshall Mail Slot 820
Little Rock, AR 72202
phone- 501.364.4240
fax- 501.364.3912
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, November 07, 2005 2:44 PM
To: LINDA MARGRAF; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] histologist/pathologist ratio
Hi Beatrice:
Some months ago I reviewed different sources (US Dept Labor; US Dept.
Commerce;ASCP) and got to the figure of about 1.4 to 2.0 histotechs per pathologist. This, as any other average, is "not written in stone" and has to vary between laboratories. Of the laboratories I personally know the averages have been from 1 to 1, to 2.2 to 1 These figures will also depend on the type of laboratory: laboratories in small hospitals will probably will be closer to 1:1, in large reference laboratories, although will have many more pathologists, the huge sample volume will require the larger side of the scale (2 or 2:2 histotechs / pathologist). Rene J.
LINDA MARGRAF
wrote:
Here's a message Beatrice is having trouble posting to the list.
Please email her or send your reply to the entire list (and not me directly). Thanks Linda M (Histonet administrator)
"DeBrosse_Beatrice"
Hi!
I would like to know what the ratio of how many histologists to a Pathologist is. Does the ratio differ from hospitals to reference labs to pharmaceutical labs? Your help is greatly appreciated.
Sincerely,
Beatrice DeBrosse-Serra
HT(ASCP)QIHC
Allergan, Inc.
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5116
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------------------------------------------------------
The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you.
==============================================================================
---------------------------------
Yahoo! FareChase - Search multiple travel sites in one click.
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 24, Issue 10
****************************************
Privileged/Confidential information may be contained in this message. The information contained in this message is intended only for the use of the recipient(s) named above and their co-workers who are working on the same matter. The recipient of this information is prohibited from disclosing the information to any other party unless this disclosure has been authorized in advance.
If you are not intended recipient of this message or any agent responsible for delivery of the message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of this message is strictly prohibited. You should immediately destroy this message and kindly notify the sender by reply E-Mail. Please advise immediately if you or your employer does not consent to Internet E-Mail for messages of this kind. Opinions, conclusions and other information in this message that do not relate to the official business of the firm shall be understood as neither given nor endorsed by it.
Privileged/Confidential information may be contained in this message. The information contained in this message is intended only for the use of the recipient(s) named above and their co-workers who are working on the same matter. The recipient of this information is prohibited from disclosing the information to any other party unless this disclosure has been authorized in advance.
If you are not intended recipient of this message or any agent responsible for delivery of the message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of this message is strictly prohibited. You should immediately destroy this message and kindly notify the sender by reply E-Mail. Please advise immediately if you or your employer does not consent to Internet E-Mail for messages of this kind. Opinions, conclusions and other information in this message that do not relate to the official business of the firm shall be understood as neither given nor endorsed by it.
More information about the Histonet
mailing list