[Histonet] decalcification

[Allen, Rhonda]

Hello,=20
I wanted to put my 2 cents worth in about decacifying newborn mouse =
femurs.  At this age there should not be any calcium yet formed in the =
bones and do not need decalcifying.  I have done mouse heads at the PO =
age and I do not have any problem cutting them, and they do not need =
decalcified.

Rhonda Allen BA HT(ASCP)HTL, QIHC
Stowers Institute
Histotechnology Specialist II	=09
1000 E. 50th street
Kansas City, Missouri 64110
rra <@t> stowers-institute.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu =
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pex
Sent: Wednesday, November 02, 2005 7:52 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decalcification


Dear all,
=20
I have a question about decalcification. I have got some bone tissues=20
(such as femur) from newborn pups, I would like to know if they need to=20
be decalcified before paraffin embedding.
=20
Thanks a lot.
=20
Guofeng


	=09
---------------------------------
 =
=D1=C5=BB=A2=C3=E2=B7=D1G=D3=CA=CF=E4=A3=AD=D6=D0=B9=FA=B5=DA=D2=BB=BE=F8=
=CE=DE=C0=AC=BB=F8=D3=CA=BC=FE=C9=A7=C8=C5=B3=AC=B4=F3=D3=CA=CF=E4
 =
=D1=C5=BB=A2=D6=FA=CA=D6=A1=A7D=CB=D1=CB=F7=A1=A2=C9=B1=B6=BE=A1=A2=B7=C0=
=C9=A7=C8=C5 =20
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu =
http://lists.utsouthwestern.edu/mailman/listinfo/histonet