[Histonet] decalcification

Allen, Rhonda RRA <@t> Stowers-Institute.org
Wed Nov 2 13:18:18 CST 2005


Hello,=20
I wanted to put my 2 cents worth in about decacifying newborn mouse =
femurs.  At this age there should not be any calcium yet formed in the =
bones and do not need decalcifying.  I have done mouse heads at the PO =
age and I do not have any problem cutting them, and they do not need =
decalcified.

Rhonda Allen BA HT(ASCP)HTL, QIHC
Stowers Institute
Histotechnology Specialist II	=09
1000 E. 50th street
Kansas City, Missouri 64110
rra <@t> stowers-institute.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu =
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pex
Sent: Wednesday, November 02, 2005 7:52 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decalcification


Dear all,
=20
I have a question about decalcification. I have got some bone tissues=20
(such as femur) from newborn pups, I would like to know if they need to=20
be decalcified before paraffin embedding.
=20
Thanks a lot.
=20
Guofeng


	=09
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