[Histonet] Antibodies for frozen mouse sections

Lindsay Hall lh2 <@t> sanger.ac.uk
Wed Nov 2 11:00:20 CST 2005


Hi, I am staining some frozen mouse NALT and cervical lymph node
sections with a number of antibodies, CD4, CD8, B220, CD11c (all from
Pharmingen) and F4/80 (Serotec) and the staining is working fine (thanks
to Gayle Callis!!). However I want to start staining for some more cell
markers and I was wondering if anybody could give me details of primary
antibodies (dilutions etc) and where you get them from, that are good
for staining natural killer (NK) cells, ICAM-1 (CD54), CD5, MadCAM-1,
VCAM-1, PNAd and a neutrophil marker, i.e. Ly-6G.
 
Thanks very much in advance.
 
Lindsay Hall
Wellcome Trust Sanger Institute
Wellcome Trust Genome Campus
Hinxton
Cambridge
CB10 1SA
UK


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