[Histonet] decalcification

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Nov 2 07:56:51 CST 2005


Yes, they need to be decalcified, gently though!
If you use an acid decalcifier (like "RDO") you may want to dilute it in half and check the bones for flexibility more often than usual.
Rene J.

pex <pex0220 <@t> yahoo.com.cn> wrote:
Dear all,

I have a question about decalcification. I have got some bone tissues 
(such as femur) from newborn pups, I would like to know if they need to 
be decalcified before paraffin embedding.

Thanks a lot.

Guofeng



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