[Histonet] Speaking of Gram's...

Tue May 31 13:33:01 CDT 2005

I'm having trouble with my Gram's in that my positive bacteria are
losing color.  So, I'm wondering what the exact differentiation time
should be for the step after the Gram's Iodine (I'm using the Sigma
kit)? The instructions are not precise, so I'm thinkin' maybe I'm over
differentiating or under-Safranin-ing or aliens (this is the State
that's home to ROSWELL...) have taken over my kit.  Anyone have anything
a bit more specific?  I'd appreciate any help...

Sara A. Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
POBox 4700
Albuquerque, NM  87196
(505)841-2576
(505)841-2518 FAX