[Histonet] slide drying

[Smith, Allen]

  I have tried melting the wax. If the wax actually melts (becomes liquid),
the sections are likely to warp.
  Drying the sections for 48 hours just below the melting point of the wax
has worked best for me.  I suspect that the longer warming time denatures
the albumin and makes it a better adhesive.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Edmondson
David (RBV) NHS Christie Tr
Sent: Thursday, May 26, 2005 1:02 PM
To: Steven Coakley; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] slide drying

Has anyone suggested that the sections need melting on rather than just
drying? or have you done this in the process described.       Shaking
trapped water off before drying and then melting may be an issue if you have
trapped amounts of water under the sections. Whether melted or just dried
on.
David
Christie
Manchester UK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Steven
Coakley
Sent: 24 May 2005 19:37
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] slide drying


I'm setting up a small Histo lab and using a 60C slide warmer to dry my
slides.  I've been currently allowing them to air dry vertically for a
couple of hours to overnight, then placing the slides on the slide warmer
for an hour.  About 90% of the time the slides turn out ok, but some slide
do show some detaching from the slide.  Both charges and non-charged slides
with water bath adhesive seem to be involved.  I read a past artical on this
site not recommending allowing slides to air dry as the section may entrap
water?  Any suggestions.
 
Steve


		
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