[Histonet] (sans sujet)

[Myri37 <@t> aol.com]

BMA is butylmethacrylate, i make a mixture of Methylmethacrylate 60ml,  
butylmethacrylate 35 ml, methylbenzoate 5ml, and 1.2 ml PEG 400.
i Fix samples in paraformaldehyde 4%, then dehydrate in ethanol, clear in  
xylene, then in :
MMA 1 : mixture as below.
MMA2 : mixture + 04% of PBO (peroxyde benzoyle dried)
MMA3 : mixture + 0.8 % of PBO.
all these stpes at 4°C.
then polymerisation MMA3 + N N dimethyltolidine at -20°C.
titanium samples are disks of 1cm od diameter.
 
thanks 
Myriam Baali