[Histonet] HER-2 ICC protocol for breast cancer cell lines

[Caroline Bass]

Hello,

I am attempting to stain a couple of breast cancer cell lines for  
HER-2.  Does anyone have a protocol that I could use?  I am trying to  
avoid paraffin embedding as I am not set up to do this.  I am using  
MCF-7, BT474 and MDA-MB-453 cells.

Any help would be appreciated.  I am relatively new to  
immunostaining, so even if there is no advice for HER-2 in  
particular, I would like to hear general staining advice for these  
cell lines.  I think I have to do an antigen retrieval step, but am  
not sure how to modify the FFPE protocols that I have seen.

Thanks,

Caroline Bass