[Histonet] GFAP - Rat

[Geoff McAuliffe]

    I also have had excellent results with DAKO's rabbit anti-cow GFAP 
(Z0334). I use it with a Vector ABC Elite kit and DAB for the chromogen. 
For FFPE sections my primary AB dilutions are about 1:5000 when using 
nickel + cobalt to intensify the DAB, more concentrated without the 
nickel-cobalt.

Geoff

Bruijntjes, J.P. wrote:

>Hi all
>
> 
>
>Is anyone of you working with rat brain and GFAP? 
>
>I have noticed that many GFAP antibodies do cross react with rat tissue,
>but maybe someone of you is working with rat tissue and does have a
>preference to a particular antibody. If so, can you share this knowledge
>with me?
>
> 
>
>Joost Bruijntjes
>
>TNO Quality of Life
>
>Zeist Holland
>
> 
>
> 
>
> 
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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