[Histonet] Envrion LpH treatment of prion diseased tissues

[Gayle Callis]

Environ LpH (EPA reg No. 1043-118) via key word search into Steris website 
gave directions for use, sites handling deer, elk, beef and sheep tissues 
with  following transmissable spongioform encephalopathies  (TSEs). Chronic 
wasting disease (CWD) scrapie, bovine spongiform encephalopathy 
(BSE).  Nothing was said about treating human tissues with CJD or CJD variant.

However the following publication was worth reading on this new product as 
compared to sodium hydroxide or bleach treatments.

Race RE, Raymond GJ. Inactivation of transmissible spongiform 
encephalopathy (Prion) agens by Environ LpH J Virology 78(4):2164-2165, 
2004.  Although the testing in this publication was done using hamster 
prion model, this agent is being used by hospitals, and other institutions 
for prion disease.  Environ LpH was recommended but not other LpH products.

It will be interesting to see if this product becomes the recommended means 
for dealing with prion diseases.

Cynthia's advice is well taken AND I would be inclined to treat the 
fixative the tissue was in also.

At 10:43 AM 5/20/2005, you wrote:
>Sally,
>
>If the formic acid was done according to protocol for prion deactivation
>then theoretically you should have no problem with using an automated
>stainer. There are probably a few things to keep in mind. How was the
>specimen cut? Were precautions taken at that time? In the current animal
>models the infectious agent needs to be ingested or inoculated IC to cause
>disease. The prion is bound to the tissue which is bound to the slide so my
>feeling is that things are pretty stable at this point. Alternatively you
>could stain by hand using disposable containers, segregate your waste and
>treat with Environ LpH which inactivated prions. If you do put it on an
>automated stainer you should collect your waste and treat to deactivate
>
>c
>
>
>Cynthia Favara
>NIAID/NIH/RML/LPVD
>903 South 4th Street
>Hamilton, MT 59840
>406-363-9317
>
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>
>-----Original Message-----
>From: Drew Sally A. [mailto:sa.drew <@t> hosp.wisc.edu]
>Sent: Friday, May 20, 2005 9:17 AM
>To: Histonet (Histonet)
>Subject: [Histonet] Forgot subject line before-Immunostains on suspected CJD
>
>How do people deal with immuno requests on formic acid treated autopsy
>tissue from
>suspected CJD cases?  Are there concerns with putting these slides
>through
>automated immunostainers?
>
>
>Sally Ann Drew, MT(ASCP)
>IHC/ISH Laboratory
>University of Wisconsin Hosp. & Clinics
>Madison, WI 53792
>(608)265-6596
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)