[Histonet] Double fluorescent stains for apoptosis

[Kelly D Mcqueeney]

The reason people subscribe to histonet is to reduce time spent "going 
to the library and spending half a week immersing yourself in the 
published literature of cell death and the methods used to recognize how 
it happens." This is achieved by asking your peers and "experts" for 
help. You should copy and send this response to everyone asking advice 
when they can simply take a week and look it up. I know I don't have 
that kind of time, and it is clear that Chan Wai Kam does not either.

Chan Wai Kam:  I would help you, my friend, but I do not have experience 
with the Roche kit.

Kelly McQueeney
Research Scientist
Bristol-Myers Squibb

John Kiernan wrote:

>Dear Chan Wai Kam,
>
>Your enquiry clearly indicates that you don't know how
>your bought kit works. The TUNEL (not tunnel! It's an
>acronym for Terminal Uridine Nick-End Labelling) family 
>of methods has many shortcomings, all thoroughly 
>documented in peer-reviewed papers that are cited in
>textbooks. TUNEL, intelligently used, has its place, 
>alongside other techniques that indicate modes of
>cell death. 
>
>Your "we need a protocol" plea is a way of saying 
>"Tell me exactly what to do" instead of going to the
>library and spending half a week immersing yourself
>in the published literature of cell death and the
>methods used to recognize how it happens. 
>
>Are you a clinical resident required to do a research
>project in a few weeks? This is the impression that
>I get from your email. The only sensible reply is
>"Go to the library and tell your boss to go there 
>too".
>  
>