[Histonet] immunofluorescence background!!

[pex]

At first, I will thank Dr. Gayle and Dr. Bob for their good suggestions.
But I find that there are so many background and so many spots due to the problems of primary antibodies possibly, because there is seldom backgound in my negative sections.Recently I check some data about reducing background, but many data only show the common causes of background from secondary antibodies alone,  so I have no ideas about primary antibodies. In addition, I am sure my primary antibodies are good, they come from polysciences and acris. others have used them to get good results.
Next time I will try a method from Dr.Bob. But I am not sure if it is OK.
 
 
Guofeng



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