[Histonet] RE: MSc Histopathology

[Ahmed, T (Tanni)]

Dear Sunni and histonetters,

Re: postgraduate histology training.

I am intending to start a part time Histopathology postgraduate Msc
course with Imperial College uni (imperial.ac.uk) this coming October. I
am currently working for a veterinary co'(who are sponsoring and funding
the course), where I have been setting up and running the histolab. I
had previously worked as a trainee in a private healthcare company, also
in histopath - (human histology) for one year after completing my degree
in Biochemistry, two years ago. I decided the NHS training didn't suit
what I wanted to do...it is a grading system here in the UK, where you
are trained in various areas such as immunology, heamatology etc.
However, you require a Biomedical Science degree to start off with - the
Institute of BMS would not accept my degree, unless I did a top-up
post-grad diploma and worked in an NHS lab for 2 years...so I took plan
B, the backdoor....

Initially I was unsure whether to go ahead with a postgrad - in terms of
becoming specialised in that particular field, however it made sense as
my degree only had one histology module. I think a taught course like
this, would be ideal, providing a thorough foundation in the fundamental
mechanisms underpinning pathology, both practically and theoretically.
The majority of students will predominantly be from the NHS, with human
histology being the core area of teaching. However, I believe the
fundamentals of histology is similar for both human/animal histopath and
the course is also designed to accommodate applicants in the research
field.   

The course entry requirements are an upper second class degree or above
here, however they often take students who have lower qualifications if
we feel that they are suitable. The course is always run on a day
release basis - therefore you can get best of both worlds, studying and
working fulltime.  The next two years are going to be hectic, but I hope
all worth it in the end as I will definetely get a lot out of it. The
course has been and is currently very successful being run alongside a
Clinical Cytology course here and they are always looking to improve the
course and keep it relevant and up-to-date for techniques etc.  All
students have to complete a laboratory-based project of their choice and
are expected to have a local supervisor and deal with Ethics etc
although they will help wherever we can. Im considering doing a avian
related project...this may change in 5 months though!

Hope this helps....

Tanni



Tanni S Ahmed
Scientific Officer - Histopathology, R&D
Intervet UK Ltd.
Walton Manor, 
Walton, Milton Keynes,
Buckinghamshire, 
MK7 7AJ, UK.
Tel. +44(0)1908 685552/685543
Fax +44(0)1908 685614
E-mail: tanni.ahmed <@t> intervet.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: 11 May 2005 16:14
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 18, Issue 14


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Today's Topics:

   1. Mast cell stain (Osborn, Sharon)
   2. Slide labelers (Osborn, Sharon)
   3. Re: Asbestos studies (Gayle Callis)
   4. Control for Mast cells (Gayle Callis)
   5. Picrosirius red stain (Jeffrey Wu)
   6. RE: Control for Mast cells (Jim Staruk)
   7. Re: mast cell in guinea pig (John Kiernan)
   8. RE: Asbestos studies (Shirley Powell)
   9. Final notice: Missouri Society for Histotechnology	Spring
      Symposium (Johnson, Teri)
  10. Master's of Histology (Farley, Sunni R)
  11. job (cynthia haynes)
  12. Re: Slide Labeller (MajorFocus <@t> aol.com)
  13. Re: Mast cell stain (Laurie Reilly)
  14. Re: job (Patti Loykasek)
  15. Mast Cell/Giemsa clarification (HSRL)
  16. RE: Master's of Histology (Kemlo Rogerson)
  17. (no subject) (eswary)
  18. EDTA (Bernadette Weston)
  19. macrophages in mouse FFPE
      (wasielewski.reinhard.von <@t> mh-hannover.de)
  20. Gayle - floating petri dish snap freezing question
      (Kristen Broomall)
  21. RE: macrophages in mouse FFPE (Flynn, Evelyn)
  22. New rabbit antibody... but what do I use as negative
      (isotype) control? (- -)
  23. RE: job (Margaryan, Naira)
  24. Unsubscribe (Flores, Teresa)
  25. Unsubscribe (Flores, Teresa)
  26. Help!!! (vsailes <@t> nd.edu)
  27. job listing (LINDA MARGRAF)


----------------------------------------------------------------------

Message: 1
Date: Tue, 10 May 2005 13:14:25 -0400
From: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>
Subject: [Histonet] Mast cell stain
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<29B25753F6B1D51196110002A589D44402397F8E <@t> PALMSG30.us.schp.com>
Content-Type: text/plain

Tom,
	
Luna's Toluidine Blue Method for Mast Cells is a superior demonstration
of mast cells using a pink background.  These literally pop up under low
power. The method is located on pages 311-312 of Luna's Histopathologic
Methods and color Atlas of special Stains and Tissue Artifacts; 1992.
If you wish a copy of the procedure, private email me and I will send to
you.

sharon osborn
DNAX ScheringPlough BioPharma
Palo Alto, CA

Subject: [Histonet] Giemsa Stain for Mast Cells
Netters,
I am looking for a Giemsa stain for Mast Cells that does not have a
pinkish hue like the Gaffney Giemsa.  We are looking to have the Mast
Cells pop out at us on low power.  Any suggestions/protocols? Thanks,
Tom Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract
Laboratory 137 South Main Street Woodstock, Virginia  22664
(540)459-8211
Fax: (540)459-8217
tomgalati <@t> hsrl.org
www.hsrl.org <www.hsrl.org> 



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------------------------------

Message: 2
Date: Tue, 10 May 2005 13:21:48 -0400
From: "Osborn, Sharon" <sharon.osborn <@t> dnax.org>
Subject: [Histonet] Slide labelers
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<29B25753F6B1D51196110002A589D44402397F8F <@t> PALMSG30.us.schp.com>
Content-Type: text/plain

Cynthia,
	Contact your Leica representative and ask for a demonstration of
their slide printers.  I think you will be pleasantly surprised as to
the amount of information and quietness.  I have used several different
slide labelers including the etch-a-sketch types and ink printer types;
this one is the best of all I have used.  Its footprint is a little
larger than the others and well worth it.  Sakura and Leica partnered on
developing these; their main difference is in the software that is
provided. Sakura uses off the shelf software while Leica specifically
built the software on theirs and it is customizable for stand alone or
for integration with MIS of an institution.  And, the company service
will also make a difference. 
	So, I encourage you to demo several different types of slide
labelers and find the one that best suits your needs. Sharon Osborn DNAX
ScheringPlough BioPharma Palo Alto, CA 


Date: Tue, 10 May 2005 10:37:23 -0400
From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
Subject: [Histonet] slide labeller
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<F76C9B2DA2FC4C4CA0A18E288BBCBCF70A8DB35B <@t> nihexchange24.nih.gov>
Content-Type: text/plain

I am interested in an automated slide labeler. I would like the capacity
to do multiple slides with the same information as quiet as possible and
of course I have limited space. Any suggestions would be appreciated.

 

I also have tops to the bottle for a Ventana special stainer. I am happy
to send to anyone interested.

 

c

 

Cynthia Favara 
NIAID/NIH/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
406-363-9317 



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This message and any attachments are solely for the intended recipient.
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-- Please immediately and permanently delete.



------------------------------

Message: 3
Date: Tue, 10 May 2005 11:25:43 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Asbestos studies
To: mtitford <@t> aol.com, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20050510112352.01b41688 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

If I remember correctly, an iron stain will identify asbestos fibers
nicely 
in a section.  Histonet had discussion on asbestos fibers some time ago,

may be worth a search in archives at www.histosearch.org

At 09:52 AM 5/10/2005, you wrote:
>Fred Underwood asks about asbestos fibers in lung tissues.
>
>Asbestosis and mesothelioma studies are quite big business along the 
>Gulf
>Coast here with the local shipyards and men of the "Golden Generation" 
>passing away. A lot of lawyers have made a lot of money and most
recently, 
>a big lawsuit in Corpus Christi Texas accuses screening companies of 
>misdiagnosis.
>However, onto things histological: asbestos fibers can be easily seen
in 
>an H&E section as brown, cylindrical, and beaded. In our laboratory we 
>digest lung tissue in bleach and then filter the remains through a 
>millipore. Once dried, it can be placed on a slide, the filter
dissolved 
>in chloroform and mounted with a coverslip. The fibers are then
counted.
>The reference is Roggli VL, Oury, TD and Sporn TA " Pathology of 
>asbestos-associated diseases" 2ed edition Spinger. 2004
>
>Mike Titford
>USA Pathology
>Mobile AL USA
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)





------------------------------

Message: 4
Date: Tue, 10 May 2005 11:28:28 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Control for Mast cells
To: "Anna Inman" <Anna.Inman <@t> stmarygj.org>,
	Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20050510112558.01b62930 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Skin contains mast cells in connective tissues and have seen them in 
connective tissues surrounding bones.

At 10:15 AM 5/10/2005, you wrote:
>-->
>
>  I am having difficulty getting a control for Mast cells. Any 
> suggestions?
>
>
>
>Anna Inman B.S., HT (ASCP)
>
>SMH Pathology
>
><mailto:Anna.Inman <@t> stmarygj.org>Anna.Inman <@t> stmarygj.org
>
>
>
>
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
>Sent: Tuesday, May 10, 2005 8:51 AM
>To: histosci <@t> shentel.net; Histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Giemsa Stain for Mast Cells
>
>
>
>Carsons pH 4.3 toluidine blue stain is excellent for Mast cells as is
>
>Churukian Shenk method for mast cells.  You don't have to worry about 
>so
>
>many other colors from Giemsa.  If you want these, I will be happy to
>
>attach privately.
>
>
>
>At 07:45 AM 5/10/2005, you wrote:
>
> >Netters,
>
> >
>
> >
>
> >
>
> >I am looking for a Giemsa stain for Mast Cells that does not have a
>
> >pinkish hue like the Gaffney Giemsa.  We are looking to have the Mast
>
> >Cells pop out at us on low power.  Any suggestions/protocols?
>
> >
>
> >
>
> >
>
> >Thanks,
>
> >
>
> >
>
> >
>
> >Tom
>
> >
>
> >
>
> >
>
> >Tom Galati
>
> >
>
> >Laboratory Director
>
> >
>
> >HSRL, Inc.- A GLP Compliant Contract Laboratory
>
> >
>
> >137 South Main Street
>
> >
>
> >Woodstock, Virginia  22664
>
> >
>
> >(540)459-8211
>
> >
>
> >Fax: (540)459-8217
>
> >
>
> >tomgalati <@t> hsrl.org
>
> >
>
> >www.hsrl.org
>
> >
>
> >
>
> >
>
> >_______________________________________________
>
> >Histonet mailing list
>
> >Histonet <@t> lists.utsouthwestern.edu
>
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>Gayle Callis
>
>MT,HT,HTL(ASCP)
>
>Research Histopathology Supervisor
>
>Veterinary Molecular Biology
>
>Montana State University - Bozeman
>
>PO Box 173610
>
>Bozeman MT 59717-3610
>
>406 994-6367 (lab with voice mail)
>
>406 994-4303 (FAX)
>
>
>
>
>
>
>
>_______________________________________________
>
>Histonet mailing list
>
>Histonet <@t> lists.utsouthwestern.edu
>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>This electronic message and all contents contain information from St.
>Mary's Hospital  which may be attorney-client privileged, confidential
or 
>otherwise protected from disclosure.  The information is intended to be

>for the addressee only.  If you are not the addressee, any disclosure, 
>copy, distribution or use of the contents of this message is 
>prohibited.  If you have received this electronic message in error,
please 
>notify the sender immediately and destroy the original message and all 
>copies.  Thank you

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)





------------------------------

Message: 5
Date: Tue, 10 May 2005 13:30:54 -0400
From: "Jeffrey Wu" <wurochi <@t> hotmail.com>
Subject: [Histonet] Picrosirius red stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY101-F3227F47CA5C3582A0340E0B21F0 <@t> phx.gbl>
Content-Type: text/plain; format="flowed"


   Hello all,

   I  am  trying  to  quantitate  the amount of fibrosis in mouse
cardiac
   tissue.   I  based  my  method  on  Dr Kiernan's excellent tutorial
on
   picrosirius  red  staining  (posted  in  2000).   Briefly,  slides
are
   deparaffinated  in  xylene  (2x, 6 min); 100% ethanol (2x, 3 min);
75%
   ethanol  (1x,  3 min); and running distilled water (10-15 min).
Next,
   they  are stained  in  saturated  picrosirius  red  for 60 minutes
and
   washed in acetic acid (2x, 3 min).  They are dehydrated in 75%
ethanol
   (1x,  3  min);  100%  ethanol  (2x,  3  min);  and xylene (1x, 3
min).
   Finally, the slides are mounted with Permount.

   For  image  processing,  I am using circularly polarized light.
Under
   the  microscope,  the  collagen  stains  from pink to dark red
(almost
   black),  depending  on  its  content;  however,  the background
tissue
   appears  green/blue.    It  is  problematic  because  I  quantify
the
   collagen  using  ImagePro  Plus  software,  in  which I select
certain
   colors.  Especially  with  the  pink  and  some  dark  blues, there
is
   overlap  between  the  collagen  and tissue, causing overestimation
of
   collagen.  (Sorry  for  the  long explanation.)   I  guess what  I
am
   seeking  is the  flaw in my procedure, causing the other tissue not
to
   stain yellow.

   On  a side note, I am using the correct picrosirius red stain, and
the
   solution   is   saturated   with  crystals  at  the  bottom.   I
have
   tried modifying  washing  times without any change.  I used a short
(2
   min)  0.2%  phosphomolybdic  acid  wash,  which  is supposed to
reduce
   background, with no success as well.

   Once  again,  sorry  for  the long question.  Any help with my
problem
   would be greatly appreciated.  Please do not hesitate to email me
with
   any  questions  or  anything that I have not made clear.  Thank you
in
   advance.

   J Wu


------------------------------

Message: 6
Date: Tue, 10 May 2005 13:52:27 -0400
From: "Jim Staruk" <jstaruk <@t> masshistology.com>
Subject: RE: [Histonet] Control for Mast cells
To: "'Anna Inman'" <Anna.Inman <@t> stmarygj.org>,
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0IGA002BACE3E8G4 <@t> vms048.mailsrvcs.net>
Content-Type: text/plain; charset=us-ascii

Anyone in diagnostic veterinary services gets mast cell tumors all of
the time.  I'll be glad to share some with someone in need (and I won't
even charge you).

Jim

______________________
    Jim Staruk
Mass Histology Service
www.masshistology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Tuesday, May 10, 2005 1:28 PM
To: Anna Inman; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Control for Mast cells

Skin contains mast cells in connective tissues and have seen them in 
connective tissues surrounding bones.

At 10:15 AM 5/10/2005, you wrote:
>-->
>
>  I am having difficulty getting a control for Mast cells. Any 
> suggestions?
>
>
>
>Anna Inman B.S., HT (ASCP)
>
>SMH Pathology
>
><mailto:Anna.Inman <@t> stmarygj.org>Anna.Inman <@t> stmarygj.org





------------------------------

Message: 7
Date: Tue, 10 May 2005 13:58:36 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] mast cell in guinea pig
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4280F64C.CD1F5085 <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii



John Kiernan wrote:
> 
> Probably you didn't see any mast cells in the
> sections of guinea-pig tissues because there
> were none to be seen.
> 
> Mouse and rat mast cells are unusual in that their
> granules are preserved and rendered insoluble by
> aqueous fixatives such as buffered formaldehyde.
> In most other species aqueous fixatives dissolve
> out the mast cell granules. Alcoholic fixatives (eg
> Carnoy, or the "alcoholic Bouin" fluids: Dubosq, Gendre
> etc) will preserve and insolubilize mast cell granules
> of any species.
> 
> For more on this, see "The Mast Cells" by Hans Selye
> (1965) Chapter 3. Also the "Notes & Queries section in
> the current issue of Biotechnic & Histochemistry (Vol 80
> No 1, p.43-45). The latter is available at the publisher's web site:
>
http://journalsonline.tandf.co.uk/app/home/journal.asp?wasp=5a0cd1389c12
4b68b94584a9e888d3f3&referrer=parent&backto=searchpublicationsresults,1,
1;homemain,1,1;
> 
> The metachromasia is due to heparin, the major
> macromolecular anion of the granules. The staining
> properties of heparin are similar to those of
> cartilage matrix. Both materials carry a lot
> of sulphate-ester groups. Unfortunately heparin
> (except that of rats and mice) is water-soluble.
> I do not know if any tryptase or chymase immunoreactivity remains in 
> mast cells whose granules have been extracted by an aqueous fixative.
> --
> -------------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London,   Canada   N6A 5C1
>    kiernan[AT]uwo.ca
>    http://publish.uwo.ca/~jkiernan/
>    http://instruct.uwo.ca/anatomy/530/index.htm
> _______________________________
> Elizabeth Chlipala wrote:
> >
> > Hello All
> >
> > I'm looking for a stain that will identify mast cells in guinea 
> > pigs.  I have run both giemsa (modified dif quick) and 0.4% 
> > Toluidine Blue.  I normally get very good staining of mast cells 
> > with the toluidine blue. I normally run this stain on mouse and rat 
> > tissue.  This is the first time I have tried this stain in guinea 
> > pigs.  Upon review of the slides I could not located one mast cell 
> > in 11 lung sections.  I know they have to be there.  Does the 
> > metachromatic nature of the t. blue stain have to do with the amount

> > of histamine present in the cells?  I have read in the literature 
> > that guinea pigs and humans have less histamine present in their 
> > mast cells than rats, hamsters and mice.  Is anyone aware of a 
> > modification that will stain mast cells in guinea pig?  Any help 
> > would be appreciated.  I would prefer to stick with a histochemical 
> > method. I'm aware of Immunohistochemical staining for mast cell 
> > tryptase, but in researching this I could not find any references 
> > for guinea pig tissue.
> >
> > Thanks in advance.
> >
> > Liz
> >
> > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> > Manager
> > Premier Laboratory, LLC
> > P.O. Box 18592
> > Boulder, Colorado 80308
> > Office: (303) 735-5001
> > Fax: (303) 735-3540
> > liz <@t> premierlab.com
> > www.premierlab.com
> >
> > Ship to Address:
> > Premier Laboratory
> > University of Colorado
> > MCDB, Room A3B40
> > Boulder, Colorado 80309
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________



------------------------------

Message: 8
Date: Tue, 10 May 2005 14:15:25 -0400
From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
Subject: RE: [Histonet] Asbestos studies
To: 'Gayle Callis' <gcallis <@t> montana.edu>, mtitford <@t> aol.com,
	Histonet <@t> lists.utsouthwestern.edu
Message-ID: <01LO3GS2JNLI8WX3KK <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii

The histonet archives can be found at www.histosearch.com.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Tuesday, May 10, 2005 12:26 PM
To: mtitford <@t> aol.com; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Asbestos studies

If I remember correctly, an iron stain will identify asbestos fibers
nicely in a section.  Histonet had discussion on asbestos fibers some
time ago, may be worth a search in archives at www.histosearch.org

At 09:52 AM 5/10/2005, you wrote:
>Fred Underwood asks about asbestos fibers in lung tissues.
>
>Asbestosis and mesothelioma studies are quite big business along the
>Gulf Coast here with the local shipyards and men of the "Golden
Generation"
>passing away. A lot of lawyers have made a lot of money and most 
>recently, a big lawsuit in Corpus Christi Texas accuses screening 
>companies of misdiagnosis.
>However, onto things histological: asbestos fibers can be easily seen 
>in an H&E section as brown, cylindrical, and beaded. In our laboratory 
>we digest lung tissue in bleach and then filter the remains through a 
>millipore. Once dried, it can be placed on a slide, the filter 
>dissolved in chloroform and mounted with a coverslip. The fibers are
then
counted.
>The reference is Roggli VL, Oury, TD and Sporn TA " Pathology of
>asbestos-associated diseases" 2ed edition Spinger. 2004
>
>Mike Titford
>USA Pathology
>Mobile AL USA
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 9
Date: Tue, 10 May 2005 14:06:06 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Final notice: Missouri Society for Histotechnology
	Spring Symposium
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.244.1115823957.6247.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

The Missouri Society for Histotechnology Spring Symposium

When: May 20-21, 2005

Where: 
The Lodge of the Four Seasons, Lake Ozark, Missouri
(800) 843-5253
www.4seasonsresort.com

Note:  Rooms for 'The Lodge' may be fully booked so here are 2
alternative places to stay: The Country Club (800) 964-6698
(approximately 1.5 miles away) The Resort at Port Arrowhead (800)
532-3575 (approximately 3 miles away)

For additional meeting information please contact:
Sharon Walsh
Phone: (636) 305-9650
email: userwalsh <@t> aol.com
or 
Rosetta Barkley
Phone: (913) 588-2737
email: rbarkley2 <@t> kumc.edu


We invite you to participate in a unique learning experience.  The
program is outstanding this year and should have something for everyone.
In addition there will be social activities and vendor displays.

Please Note: All workshops are CEU approved.

Friday Schedule:
  8:30am "Ergonomics in the Workplace", Jan Minshew, Leica Microsystems
  9:15am "Case Studies in Forensic Pathology", Dr. Michael Graham, St.
Louis University 10:45am "Histochemistry of Special Stains", Jerry
Fredenburgh  1:30pm-4:30pm Workshops #1, #2, or #3 (Your choice)
6:00-8:00pm - Cruise on the "Lake of the Ozarks" Come enjoy the lake and
visit with exhibitor's and friends.

Saturday Schedule:
 8:15am "Creutzfeldt-Jakob Disease: Tissue Handling and Testing in the
United Kingdom", Konnie Zietner, Nebraska Medical Center  9:15am
"Histological Techniques in Hematopathology", Dr. Sharad Mather 10:45am
"Applications for Microwave Decalcification Procedures", Skip Brown
1:30pm-4:30pm Workshops #4, #5, or #6 (Your choice)

Registration Fees: 
Members - $50,  Non-Members - $60
Additional Banquet Ticket - $25

2005 WORKSHOP DESCRIPTIONS

Workshop#1
Theory of Routine & Microwave Fixation & Processing - Skip Brown & Jerry
Fredenburgh The process of tissue fixation and processing will be
illustrated; first from a molecular level to demonstrate what is
actually happening at a micro level in the tissue; then from a macro
level to show the gross effects on the tissue.  Accelerated procedures
will be introduced as a way to speed up the process without detrimental
effects to the tissue.

Workshop #2			(Sponsored by Ventana Medical Systems)
 A Tour Guide to Immunohistochemistry: How to get there and what to do
when you arrive. - Chris Moore, BS World Wide Project Manager, Special
Stains, Ventana Medical Systems, Inc. Immunohistochemistry is defined
as, "A method of detecting the presence of specific proteins in cells or
tissues." If only it was that easy. A better definition may be, "A
method of standardizing each and every step in and out of your control
working toward a complex chemical construction of molecules to identify
specific proteins in a variety of tissues in a variety of stages in
order to answer specific questions." This class will not only identify
the building blocks of immunohistochemistry, from rabbit monoclonal
antibodies to polymer detection kits, but we will also review how each
step before the staining can affect those building blocks. This class
will also address some basic dilution techniques for concentrated
antibodies, the use of positive and negative controls, where to start
trouble shooting your new antibody. Finally, we will discuss the utility
of IHC in the clinical and research setting and its relevance to drug
discovery and disease treatment. 

Workshop #3
Immunocytochemical and Immunohistochemical assays. A Pathologist's
perspective, Lourdes R. Ylagan MD, Washington University. This workshop
will show a brief overview of both immunohistochemical and
immunocytochemical techniques. It will also show examples of how double
immunostaining techniques can be helpful in both histologic and
cytologic samples. It will also show the ways in which immunochemistry
is used in: (1) the elucidation of the site of origin of a poorly
differentiated neoplasm in the setting of a metastasis, (2) detection of
antigens present in the surface of tumor cells amenable to
antibody-mediated chemotherapeutic agents, (3) detection of tumor
markers known to be potential poor prognostic indicators in tumors.

Workshop #4			(Sponsored by Leica Microsystems)
Quality and Skill in Microtomy Technique - Jan Minshew, Leica
MicroSystems This session will present an understanding of the physical
dynamics of microtomy, and discuss the effects of external variables
such as knife angle, room temperature, inadequate instrumentation, etc.

Workshop #5			(Sponsored by Ventana Medical Systems)
Special Stains: the Once and Future King? Chris Moore, BS World Wide
Project Manager, Special Stains, Ventana Medical Systems, Inc. Special
Stains are an art of using dyes to stain specific tissues and/or
cellular structures that were once thought of as a major advancement in
the histology lab. Now we have much more complex chemicals to stain
proteins and genes within those cells and tissues, yet we still find a
vast usage of special stains in the histology lab. In fact, some of the
specials stains have become so routine that they are oftentimes
considered as routine as the H&E in certain circumstances. Why do we
still utilize special stains when we have far less hazardous chemicals
that can pinpoint much more specific things? This class will address
that question. We will discuss where special stains has been, why they
have been around for so long, and where we see them going. This class
will break down the most frequently used special stains; discuss their
bio-chemical reactions and their utility. We will then compare those
special stains to their relevant immunohistochemistry and in-situ
hybridization counterparts to illustrate their utility and futility .  
Workshop #6
>From Bench Tech to Management, Konnie Zietner, Nebraska Medical Center
Basic principles and skills you will need when transitioning from
technician to management.





------------------------------

Message: 10
Date: Tue, 10 May 2005 12:34:23 -0700
From: "Farley, Sunni R" <sfarley <@t> seattlecca.org>
Subject: [Histonet] Master's of Histology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<5E6BFDF4F0AB2C4DA69CF4473FC7B948323349 <@t> wala01.seattlecca.org>
Content-Type: text/plain;	charset="ISO-8859-1"

I have recently learned that you can earn a Master's of Histology/Cell
Biology - does anybody have any information and/or recommendations on
earning this degree?  Thank you ahead for your time!

Sunni



Sunni R. Farley
Histotechnician
Clinical Pathology 
Mailstop G1-201

Seattle Cancer Care Alliance
825 Eastlake Ave East
Seattle, WA 98109
(206) 288-1312



 This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the intended
recipient, be aware that any disclosure, copying, distribution or use of
the contents of this information is prohibited. If you have received
this electronic transmission in error, please leave a message via
telephone at (206) 288-6266, notify me by electronic reply, and delete
this message. Opinions and ideas in this message that do not relate to
official business are understood as neither given nor endorsed by the
Seattle Cancer Care Alliance. To view our complete Notice of Privacy
Practices, visit our web site at www.seattlecca.org.




------------------------------

Message: 11
Date: Tue, 10 May 2005 14:54:16 -0700 (PDT)
From: cynthia haynes <naje1972 <@t> yahoo.com>
Subject: [Histonet] job
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050510215416.51974.qmail <@t> web41710.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hi! 

We are looking for people who might be interested in a
pool position in the Chicago land area. 
Please contact me Cynthia Haynes at 1-773-484-4133.

Thanks in Advance

Cynthia Haynes H.T.


		
__________________________________ 
Yahoo! Mail Mobile 
Take Yahoo! Mail with you! Check email on your mobile phone. 
http://mobile.yahoo.com/learn/mail 



------------------------------

Message: 12
Date: Tue, 10 May 2005 18:56:59 EDT
From: MajorFocus <@t> aol.com
Subject: [Histonet] Re: Slide Labeller
To: cfavara <@t> niaid.nih.gov
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1f4.978eaa2.2fb2963b <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

I have used the Thermo Microwriter as well which is quite nice.  If  you
are 
interested in an inexpensive software program for generating slide
labels, 
check out our website for the Histo-Labels program.  It will also
interface 
with most slide and cassette printers/etchers as well.
_www.majorfocus.com_ (http://www.majorfocus.com) 
 
Greg L. Good, HT(ASCP)
Major Focus
(800) 888-1152


------------------------------

Message: 13
Date: Wed, 11 May 2005 09:36:52 +1000
From: Laurie Reilly <laurie.reilly <@t> jcu.edu.au>
Subject: Re: [Histonet] Mast cell stain
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5.1.0.14.0.20050511092655.019a6ec0 <@t> mail.jcu.edu.au>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Hi Histonetters,
We routinely stain all dog skin lesions with Toluidine Blue and it 
highlights the Mast Cells.
They stain metachromatically and show up with red-purple granules in the

cytoplasm.
These biopsies are all fixed in 10% Neutral Buffered Formalin. The
method we use is from Gretchen Humason, "Animal tissue Techniques", 3rd 
edition.
It uses a solution of 0.2% Tol Blue in 60% Ethanol for 2 minutes.

   Regards,  Laurie.

At 01:14 PM 05/10/05 -0400, Osborn, Sharon wrote:
>Tom,
>
>Luna's Toluidine Blue Method for Mast Cells is a superior demonstration

>of mast cells using a pink background.  These literally pop up under 
>low power. The method is located on pages 311-312 of Luna's 
>Histopathologic Methods and color Atlas of special Stains and Tissue 
>Artifacts; 1992.  If you wish a copy of the procedure, private email me

>and I will send to you.
>
>sharon osborn
>DNAX ScheringPlough BioPharma
>Palo Alto, CA
>
>Subject: [Histonet] Giemsa Stain for Mast Cells
>Netters,
>I am looking for a Giemsa stain for Mast Cells that does not have a 
>pinkish hue like the Gaffney Giemsa.  We are looking to have the Mast 
>Cells pop out at us on low power.  Any suggestions/protocols? Thanks,
>Tom
>Tom Galati
>Laboratory Director
>HSRL, Inc.- A GLP Compliant Contract Laboratory
>137 South Main Street
>Woodstock, Virginia  22664
>(540)459-8211
>Fax: (540)459-8217
>tomgalati <@t> hsrl.org
>www.hsrl.org <www.hsrl.org>
>
>
>
>*********************************************************************
>This message and any attachments are solely for the intended recipient.

>If
>you are not the intended recipient, disclosure, copying, use or 
>distribution of the information included in this message is prohibited
-- 
>Please immediately and permanently delete.
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Mr.Laurie Reilly                                              Ph 07 4781
4468
School of Veterinary & Biomedical Science      Fax  07 4779  1526
James Cook University
Townsville  Qld. 
4811                                      laurie.reilly <@t> jcu.edu.au 

Australia.




------------------------------

Message: 14
Date: Tue, 10 May 2005 16:41:50 -0700
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: Re: [Histonet] job
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <BEA694CE.8BA0%ploykasek <@t> phenopath.com>
Content-Type: text/plain; charset="US-ASCII"

You can count me in if you mean moving to a warmer climate, more
sunshine, and definite pool/sun time!

Patti Loykasek
Seattle, WA


> Hi!
> 
> We are looking for people who might be interested in a
> pool position in the Chicago land area.
> Please contact me Cynthia Haynes at 1-773-484-4133.
> 
> Thanks in Advance
> 
> Cynthia Haynes H.T.
> 
> 
> 
> __________________________________
> Yahoo! Mail Mobile
> Take Yahoo! Mail with you! Check email on your mobile phone. 
> http://mobile.yahoo.com/learn/mail
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet







------------------------------

Message: 15
Date: Tue, 10 May 2005 20:32:13 -0400
From: "HSRL" <histosci <@t> shentel.net>
Subject: [Histonet] Mast Cell/Giemsa clarification
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <000601c555c0$e5a31840$0200a8c0 <@t> HSRLMAIN>
Content-Type: text/plain;	charset="US-ASCII"

Netters,

 

I agree with all of you about the Tol Blue stain being a good mast cell
stain.  However, we are specifically looking for a Giemsa stain for mast
cell granules.  We already do the Tol Blue, and the Pinacynol
Erythrosinate stains for mast cells. 

 

Thanks again,

 

 

Tom Galati

Laboratory Director

HSRL, Inc.- A GLP Compliant Contract Laboratory

137 South Main Street

Woodstock, Virginia  22664

(540)459-8211

Fax: (540)459-8217

tomgalati <@t> hsrl.org

www.hsrl.org

 



------------------------------

Message: 16
Date: Wed, 11 May 2005 07:42:03 +0100
From: Kemlo Rogerson <Kemlo.Rogerson <@t> elht.nhs.uk>
Subject: RE: [Histonet] Master's of Histology
To: "'Farley, Sunni R'" <sfarley <@t> seattlecca.org>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<1030B679AD69D6119C3F00080210DD9D05A3F2A7 <@t> bhrv-nt-11.bhrv.nwest.nhs.uk>
	
Content-Type: text/plain

In the UK you can obtain a Master of Science Degree by pursuing a
research project. In fact you could always take a Master's or Doctorate
if you could find a Uni, a Supervisor and something of interest to
research.

I feel that postgraduate degrees have become easier to obtain, but then
we old people always say that. In the UK NVQ's and other vocational
qualifications are becoming more popular as it is really a judgement
call, and I'm going to get into trouble, but maybe postgraduate degrees
could be thought of as an overkill for many positions in the Laboratory.
Do we really have that much to offer MSc's and PHD's? Other than funding
them to get the qualification; I fess up to having an MSc, but......  

-----Original Message-----
From: Farley, Sunni R [mailto:sfarley <@t> seattlecca.org] 
Sent: 10 May 2005 20:34
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Master's of Histology

I have recently learned that you can earn a Master's of Histology/Cell
Biology - does anybody have any information and/or recommendations on
earning this degree?  Thank you ahead for your time!

Sunni



Sunni R. Farley
Histotechnician
Clinical Pathology 
Mailstop G1-201

Seattle Cancer Care Alliance
825 Eastlake Ave East
Seattle, WA 98109
(206) 288-1312



 This electronic message transmission contains information which may be
confidential or privileged. The information is intended to be for the
use of the individual or entity named above. If you are not the intended
recipient, be aware that any disclosure, copying, distribution or use of
the contents of this information is prohibited. If you have received
this electronic transmission in error, please leave a message via
telephone at (206) 288-6266, notify me by electronic reply, and delete
this message. Opinions and ideas in this message that do not relate to
official business are understood as neither given nor endorsed by the
Seattle Cancer Care Alliance. To view our complete Notice of Privacy
Practices, visit our web site at www.seattlecca.org.


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 17
Date: Wed, 11 May 2005 17:07:14 +0800
From: "eswary" <eswary <@t> carif.com.my>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200505111707.AA254607564 <@t> carif.com.my>
Content-Type: text/plain; charset=us-ascii

hi,

does anyone know the advantage of using 0.01M sodium citrate over 0.01M
citric acid buffer, pH 6, for antigen retrieval on paraffin embedded 
sections? also, is the sodium constituent important? would it matter if 
i used trisodium citrate instead of monosodium?

thanks.

et





------------------------------

Message: 18
Date: Wed, 11 May 2005 08:31:25 -0400
From: "Bernadette Weston" <bernaweston <@t> hotmail.com>
Subject: [Histonet] EDTA
To: histonet <@t> pathology.swmed.edu
Message-ID: <BAY103-F37979F23CC16320CE78BF3A1100 <@t> phx.gbl>
Content-Type: text/plain; format=flowed

We can no longer supply EDTA for our bone marrow procedures, can some
one 
recommend a substitution for aspirate collection?

Bernadette Weston HT
Histology Supervisor
Barberton Citizens Hospital
Barberton, OH





------------------------------

Message: 19
Date: Wed, 11 May 2005 14:40:33 +0200
From: wasielewski.reinhard.von <@t> mh-hannover.de
Subject: [Histonet] macrophages in mouse FFPE
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <42821961.31188.FCDE1BE2 <@t> localhost>
Content-Type: text/plain; charset=US-ASCII

Hi Histonetters,
I am looking for a suitable antibody working in FFPE mouse tissue to
detect 
macrophages (reproducibly and reliable).

Many thanks in advance
Reinhard





PD Dr. med. Reinhard von Wasielewski




------------------------------

Message: 20
Date: Wed, 11 May 2005 08:46:42 -0400
From: "Kristen Broomall" <kbroomal <@t> NEMOURS.ORG>
Subject: [Histonet] Gayle - floating petri dish snap freezing question
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<2B9D24FC863EC841B161CBA3BA4D5B3C0181D49A <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset=iso-8859-1

Gayle,

I tried out your snap freezing method with the floating petri dish in
the liquid nitrogen yesterday for some itsy bitsy GI biopsies. I really
like it! I have a question though. When using this method, do you place
your sample in the bottom of your mold, then OCT on top, or put a dab
(or more) of OCT, then the tissue, ... or does it really matter? 

Thanks,

Kristen Broomall, HT (ASCP)




------------------------------

Message: 21
Date: Wed, 11 May 2005 09:21:33 -0400
From: "Flynn, Evelyn" <Evelyn.Flynn <@t> childrens.harvard.edu>
Subject: RE: [Histonet] macrophages in mouse FFPE
To: wasielewski.reinhard.von <@t> mh-hannover.de,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<A75ECE3D2DE43D4CB5A9F3B55177D8CAE8BA7C <@t> CHEXV2.CHBOSTON.ORG>
Content-Type: text/plain; charset=iso-8859-1

Dear Reinhard,
        I have had good results with rat anti-mouse Mac-3 antibody from
PharMingen (Cat. # 550292).

Regards,
Evelyn Flynn, M.A.
Children's Hospital, Boston



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
wasielewski.reinhard.von <@t> mh-hannover.de
Sent: Wed 5/11/2005 8:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] macrophages in mouse FFPE
 
Hi Histonetters,
I am looking for a suitable antibody working in FFPE mouse tissue to
detect 
macrophages (reproducibly and reliable).

Many thanks in advance
Reinhard





PD Dr. med. Reinhard von Wasielewski


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 22
Date: Wed, 11 May 2005 07:08:31 -0700 (PDT)
From: - - <emerald_lake77 <@t> yahoo.com>
Subject: [Histonet] New rabbit antibody... but what do I use as
	negative	(isotype) control?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050511140831.96586.qmail <@t> web31706.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hi everyone,
 
I have a new antibody that I was given.  It is a rabbit polyclonal
anti-mouse antibody.  Usually I use a specific isotype control, but I do
not have info on how this antibody was made other than the epitope(s)
that it should adhere to...  but I want a negative control and was
wondering if I can just use regular rabbit serum (diluted) as a negative
seeing that it should contain the isotype IgG I am using??? I think I am
a bit confused here.... I need assistance.
 
Can I use rabbit serum as a negative control?
What dilution do I use if that is the case?
Anything else I should know before I proceed?
 
Thank you all!!
 
 

		
---------------------------------
Discover Yahoo!
 Use Yahoo! to plan a weekend, have fun online & more.  Check it out!

------------------------------

Message: 23
Date: Wed, 11 May 2005 09:26:25 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: RE: [Histonet] job
To: "Patti Loykasek" <ploykasek <@t> phenopath.com>,	"histonet"
	<histonet <@t> pathology.swmed.edu>
Message-ID:
	
<63B8B599DE283148B92E83C78B32C15DB9164E <@t> cmhexbe2.childrensmemorial.org>
	
Content-Type: text/plain

What do you mean Pool position?

Thanks,
Naira

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Tuesday, May 10, 2005 6:42 PM
To: histonet
Subject: Re: [Histonet] job

You can count me in if you mean moving to a warmer climate, more
sunshine, and definite pool/sun time!

Patti Loykasek
Seattle, WA


> Hi!
> 
> We are looking for people who might be interested in a
> pool position in the Chicago land area.
> Please contact me Cynthia Haynes at 1-773-484-4133.
> 
> Thanks in Advance
> 
> Cynthia Haynes H.T.
> 
> 
> 
> __________________________________
> Yahoo! Mail Mobile
> Take Yahoo! Mail with you! Check email on your mobile phone. 
> http://mobile.yahoo.com/learn/mail
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet





_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The contents of this e-mail message and any attachments are private and
confidential communications intended solely for the addressee(s) named
in this message. If you are not the intended recipient of this message,
please 1) immediately notify the sender by reply e-mail and then delete
this message and its attachments and 2) do not read, use, distribute
disclose or copy this message and/or any attachments. 




------------------------------

Message: 24
Date: Wed, 11 May 2005 09:28:22 -0500
From: "Flores, Teresa" <tflore <@t> lsuhsc.edu>
Subject: [Histonet] Unsubscribe
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet-request <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu,
	"'histonet <@t> pathology.swmed.edu'" <histonet <@t> pathology.swmed.edu>
Message-ID:
	
<FFBCB2526662D411A17600B0D03D55F01369B871 <@t> lsumc-med-exch2.med.lsuhsc.edu
>
	
Content-Type: text/plain

Please unsubscribe.


------------------------------

Message: 25
Date: Wed, 11 May 2005 09:28:22 -0500
From: "Flores, Teresa" <tflore <@t> lsuhsc.edu>
Subject: [Histonet] Unsubscribe
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet-request <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu,
	"'histonet <@t> pathology.swmed.edu'" <histonet <@t> pathology.swmed.edu>
Message-ID:
	
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Content-Type: text/plain

Please unsubscribe.


------------------------------

Message: 26
Date: Wed, 11 May 2005 09:44:37 -0500
From: vsailes <@t> nd.edu
Subject: [Histonet] Help!!!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1115822677.42821a55c5ced <@t> webmail.nd.edu>
Content-Type: text/plain; charset=ISO-8859-1

Hello Histonetters,
I'm working on an IHC protocol for Albumin -- staining FFPE mouse
tissues.  I'm new to working out conditons for antibody protocols and
could use some help.I'm testing the antibody at different dilutions and
I appear to have positive staining but I'm also seeing small areas in
the negative that shows positive staining. Liver is my control and I use
steamer (HIER) for 30 minutes, my steps include a 30 min quench in 1.6%
hydrogen peroxide, NS block, Antibody, Secondary antibody(Biotin),
Streptavidin, Chromogen, Counterstain My concern is that I'm using
buffers that contain BSA and I'm wondering if that is causing staining
to appear or should I be using avidin/biotin block along with a peroxide
block. Any suggestion would be helpful. Thank you for your help in
advance. Valerie







------------------------------

Message: 27
Date: Wed, 11 May 2005 10:05:33 -0500
From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
Subject: [Histonet] job listing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s281d8fb.067 <@t> mail.childrens.com>
Content-Type: text/plain; charset=US-ASCII

Lead and/or Bench Histology Technician

Scott & White is seeking a Lead and/or Bench Histology Technician. 
Responsible for histology laboratory operations including testing,
training of support personnel, QA, personnel utilization and regulatory
compliance.  Selected candidate will also participate in routine
histology laboratory functions.   Requirements: An Associate's degree;
HT(ASCP); 3 to 5 years experience.

Just an hour north of Austin, Scott & White offers highly competitive
salaries, comprehensive benefits and advancement opportunities.
Candidates should send resumes to: ghollie <@t> swmail.sw.org. Phone:
800.527.JOBS. Fax: 254.724.5591. Apply in person or mail resume to:
Human Resources Dept., 2401 S. 31st St., Temple, TX 77508.

www.sw.org 

An equal opportunity employer/tobacco-free environment.




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End of Histonet Digest, Vol 18, Issue 14
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