[Histonet] Luna's toluidine blue; also Ventana Benchmark XT morphology

Malam Jacqueline Jacqueline.Malam <@t> rli.mbht.nhs.uk
Wed May 11 10:56:55 CDT 2005


I would appreciate it if I could also be sent details of Luna's toluidine
blue stain for mast cells, and any others that are considered superior so I
can compare with our current method. Thanks!

Also - do any of you users of Ventana's Benchmark or Benchmark XT
autoimmunostainer have a problem with poor morphology after heat retrieval -
usually affecting collagen / fibro-fatty tissues, and also the occasional
section detachment? We trialled the Benchmark which was fine but the
Benchmark XT which we purchased a year ago produces this problem. It does
not affect our positive control tissue (or very little) and I have been
through every possibility within the department and with Ventana but to no
avail. All ideas / clues welcome!
Jacqui Malam
Royal Lancaster Infirmary



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