[Histonet] New rabbit antibody... but what do I use as negative
(isotype) control?
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emerald_lake77 <@t> yahoo.com
Wed May 11 09:08:31 CDT 2005
Hi everyone,
I have a new antibody that I was given. It is a rabbit polyclonal anti-mouse antibody. Usually I use a specific isotype control, but I do not have info on how this antibody was made other than the epitope(s) that it should adhere to... but I want a negative control and was wondering if I can just use regular rabbit serum (diluted) as a negative seeing that it should contain the isotype IgG I am using??? I think I am a bit confused here.... I need assistance.
Can I use rabbit serum as a negative control?
What dilution do I use if that is the case?
Anything else I should know before I proceed?
Thank you all!!
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