[Histonet] Asbestos studies

[Gayle Callis]

If I remember correctly, an iron stain will identify asbestos fibers nicely 
in a section.  Histonet had discussion on asbestos fibers some time ago, 
may be worth a search in archives at www.histosearch.org

At 09:52 AM 5/10/2005, you wrote:
>Fred Underwood asks about asbestos fibers in lung tissues.
>
>Asbestosis and mesothelioma studies are quite big business along the Gulf 
>Coast here with the local shipyards and men of the "Golden Generation" 
>passing away. A lot of lawyers have made a lot of money and most recently, 
>a big lawsuit in Corpus Christi Texas accuses screening companies of 
>misdiagnosis.
>However, onto things histological: asbestos fibers can be easily seen in 
>an H&E section as brown, cylindrical, and beaded. In our laboratory we 
>digest lung tissue in bleach and then filter the remains through a 
>millipore. Once dried, it can be placed on a slide, the filter dissolved 
>in chloroform and mounted with a coverslip. The fibers are then counted.
>The reference is Roggli VL, Oury, TD and Sporn TA " Pathology of 
>asbestos-associated diseases" 2ed edition Spinger. 2004
>
>Mike Titford
>USA Pathology
>Mobile AL USA
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)