[Histonet] RE: Histonet Digest, Vol 18, Issue 11-methasol fast blue

Tue May 10 02:53:35 CDT 2005

We used to use it for myelin stain - can't find mention of it being same as
Luxol, but a dye called methazol fast blue can be used instead of Luxol fast
blue MBSN for myelin - same dye - different spelling?

Jacqui Malam
Royal Lancaster Infirmary
-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu] 
Sent: 09 May 2005 18:04
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 18, Issue 11

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Today's Topics:

   1. RE: Vector Red and True Blue (C.M. van der Loos)
   2. the skeletal staining (pex)
   3. methasol fast blue (Manuelle De Bock)
   4. RE: antigen retrieval/enzyme digestion (Todd Sherman)
   5. Book- Immunoenzyme Multiple Staining Methods (Cynthia A Delong)
   6. Hospital based Histology Labs (Barnhart, Tammy)
   7. RE: Hospital based Histology Labs (GUTIERREZ, JUAN)
   8. Re: Book- Immunoenzyme Multiple Staining Methods (Gayle Callis)
   9. Re: Book- Immunoenzyme Multiple Staining Methods
      (pmarcum <@t> vet.upenn.edu)
  10. RE: Hospital based Histology Labs (Sebree Linda A.)
  11. Autoflourescence Eliminator Reagent for lipofuscin in CNS,
      other cells  (Gayle Callis)
  12. Phoenix fill in (Riesen, Rebecca)
  13. RE: Hospital based Histology Labs (Kemlo Rogerson)
  14. microscope companies (pathrm35 <@t> adelphia.net)
  15. RE: Hospital based Histology Labs
      (John.Sheppard <@t> Health-Partners.org)
  16. RE: Hospital based Histology Labs (GUTIERREZ, JUAN)
  17. Re: microscope companies (RCHIOVETTI <@t> aol.com)
  18. Thanks Book ordered (Cynthia A Delong)
  19. AW: [Histonet] Hospital based Histology Labs (Gudrun Lang)

----------------------------------------------------------------------

Message: 1
Date: Mon, 09 May 2005 09:43:52 +0200
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] RE: Vector Red and True Blue
To: histonet <@t> lists.utsouthwestern.edu
Cc: bjrenquist <@t> ucdavis.edu
Message-ID: <774aad776fb4.776fb4774aad <@t> amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"

   Hi Ben,

   It's good to read that you're at least successful with the application
   of True Blue since this isn't the most easy chromogen to deal with.
   Furthermore, your choice for this turquoise-red color combination is a
   very good one, because of it's very high contrast.

   Vector Red however, isn't that strong in my hands either. You may try
   to look with a fluorescence microscope as the Vector Red reaction
   product shows a very strong (red) fluorescence upon green light
   excitation (rhodamine-filter pack). Furthermore, I have very good
   results for both light (and fluorescence microscopy) using the new
   Dako Permanent Red kit (K0640). The staining intensity is much
   higher than with Vector Red.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389
   e-mail: [1]c.m.vanderloos <@t> amc.uva.nl

   ----- Original Message -----
      From  "Ben" <bjrenquist <@t> ucdavis.edu>
      Date  Wed, 4 May 2005 11:57:08 -0700
        To  <histonet <@t> lists.utsouthwestern.edu>
   Subject  [Histonet] Vector Red and True Blue

   Hello All,

   I  have  been  attempting  some  double  labeling for fos and estrogen
   receptor
   for  about  a month with little luck.  I am using Vector Red (fos) and
   True
   Blue  (ER)  for  my  staining.   I  am  having no problems with the ER
   labeling,
   but  the fos labeling is not that clear.  I don't appear to be getting
   a
   strong reaction product from the Vector Red.  Has anyone else had this
   problem?   How  strong  should the staining be (light pink, dark pink,
   red)?  I
   have been careful to only place in xylene for a few moments before
   coverslipping and therefore don't think fading is my problem.

   I  have been thinking that my antigen retrieval may be the problem.  I
   am
   using triton X to improve antigen retrieval, but was thinking about
   microwaving.   These  are  free  floating  sections.   First  is  this
   possible to
   do with free floati! ng sectio

References

   1. mailto:c.m.vanderloos <@t> amc.uva.nl

------------------------------

Message: 2
Date: Mon, 9 May 2005 17:26:47 +0800 (CST)
From: pex <pex0220 <@t> yahoo.com.cn>
Subject: [Histonet] the skeletal staining
To: histonet <@t> pathology.swmed.edu
Message-ID: <20050509092647.69523.qmail <@t> web15510.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312

Hello, all,

Recently I do Alizarin Red and Alcian Blue staining of mouse embryo
skeletons, but I find that my results are not better due to a wrong color.
After staining, bone should be red, and cartilage should be blue, but my
results are that bone is stained purple, and cartilage is stained green,
then I change my protocol, but I get the similar results, I do not know the
reasons, I guess that bioreagents could not be good, because I make them up,
the colors of the solutions are purple and green respectively, not red and
blue.Our bioreagents are from sigma. 

If you have some experience about it, can you do me a favor?

Best wishes!

Guofeng

---------------------------------
Do You Yahoo!?
×¢²áÊÀ½çÒ»Á÷Æ·ÖÊµÄÑÅ»¢Ãâ·ÑµçÓÊ

------------------------------

Message: 3
Date: Mon, 9 May 2005 14:22:59 +0200
From: "Manuelle De Bock" <manuelle.debock <@t> UGent.be>
Subject: [Histonet] methasol fast blue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601c55491$d6e7e540$ed54bec1 <@t> ugent.be>
Content-Type: text/plain;	charset="Windows-1252"

I am trying to stain collected gastric tissue cells with Maxwell's staining
procedure (1963) but we don't have methasol fast blue in our lab. I also
can't find any links on the web where I can buy this dye. can someone help
me with this? Is it the same dye as Luxol fast blue?

Kind regards
Manuelle

Manuelle De Bock, dierenarts (DVM)
Laboratory of Veterinary Pathology 
Department of Pathology, Bacteriology and Avian Diseases
Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133
B9820 Merelbeke - Belgium
Tel 0032(0)9 264 7745
Fax 0032(0)9 264 7789

------------------------------

Message: 4
Date: Mon, 09 May 2005 08:58:31 -0500
From: "Todd Sherman" <t-sherman <@t> comcast.net>
Subject: [Histonet] RE: antigen retrieval/enzyme digestion
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <op.sqig3tqamsrlf2 <@t> equisetum>
Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-1

On Sun, 08 May 2005 12:00:26 -0500,  
<histonet-request <@t> lists.utsouthwestern.edu> wrote:

> Today's Topics:
>
>    1. RE: antigen retrieval/enzyme digestion (Patsy Ruegg)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 8 May 2005 09:42:06 -0600
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] antigen retrieval/enzyme digestion
> To: "'Till, Renee'" <TillRenee <@t> uams.edu>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <200505081542.j48FgR75001351 <@t> pro12.abac.com>
> Content-Type: text/plain;	charset="us-ascii"
>
> I have heard of it being done both ways depending on the epitope of
> interest.  In general I do HIER first and then EIER.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Till,  
> Renee
> Sent: Monday, May 02, 2005 5:55 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] antigen retrieval/enzyme digestion
>
> When  trying both on the same slides, which would go first, the antigen
> retrieval or the enzyme digestion?
>
>
> Renee' Till, HT
> Arkansas Childrens Nutrition Center
>
> 1120 Marshall
>
> Little Rock, AR
>
> 72202
>
> (501) 364-2774

Renee,

I'd tend to agree with Patsy as far as a sequence best determined  
empirically. You'll probably need to compare each protocol for each  
antigen of interest.

 From a theoretical perspective, both techniques of "unmasking" involve the

breaking of strong molecular bonds. HIER is typically designed to cleave  
the basic side-chains of amino acids on proteins previously fixed (or  
bound to each other) with x-aldehyde. Enzyme digestion is similarly  
designed to cleave proteins but at well defined amino acids which are not  
necessarily basic - it depends on the digesting enzyme and its substrate.

My guess would be that progressing from a more basic (read simple/generic)  
to a more specific cleaving sequence would be more efficient;  
consequently, treat with HIER first and enzyme digestion second. The HIER  
would open up the tissue to further access and the enzyme digestion could  
then more specifically cleave regions hindering an even more localized  
access to your target antigen. To a great extent, these success derived  
 from these sequences will be greatly influenced by the local milieu of  
your target antigen. The relative content of various amino acids in a  
given milieu may or may not be known. To that end empirical observations  
are "best" which is why mandating one particular protocol over another may  
be a bit dubious.

Good luck.
Todd

President
HistoSoft Corporation
-- 
>>>>>>>>    www.histosoft.com    <<<<<<<<
<<<<<<<< Biology In A New Form (c) >>>>>>>

------------------------------

Message: 5
Date: Mon, 09 May 2005 09:13:04 -0500
From: Cynthia A Delong <DELONG_CYNTHIA_A <@t> LILLY.COM>
Subject: [Histonet] Book- Immunoenzyme Multiple Staining Methods
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OFA9A48623.4FDE65D0-ON05256FFC.004DD396 <@t> EliLilly.lilly.com>
Content-Type: text/plain; charset="us-ascii"

Hello All,
I am looking for Chris Van de Loos's  book"Immunoenzyme Multiple Staining 
Methods". Does anyone know of where I can get a copy of this book?
I had one and someone walked off with it. 
Thank you
Cindy

Cynthia A DeLong
LRL - Corporate Center
DC0533 - 48B/3042
355 E Merrill St
Indianapolis, IN  46225
phone:  317-276-7635
fax:  317-277-6146

------------------------------

Message: 6
Date: Mon, 9 May 2005 09:25:54 -0500 
From: "Barnhart, Tammy" <Tbarnhart <@t> primecare.org>
Subject: [Histonet] Hospital based Histology Labs
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1779904B5E82D511914C00D0B793339205BFD930 <@t> exchangent>
Content-Type: text/plain;	charset="iso-8859-1"

This is a question for hospital based histo labs.  The hospital we are based
in has talked of moving our histology laboratory to an area separated from
the surgical suites.  My question is, how many hospital histology labs are
removed from the surgical suites by a significant distance?  Of those that
are separated, do you have a frozen/gross area located away from the histo
lab but close to the OR?  As many responses as possible would be
appreciated.  This will greatly facilitate our decisions for future
locations.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

Confidentiality Notice:This e-mail message is for sole use of intended
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------------------------------

Message: 7
Date: Mon, 9 May 2005 09:35:39 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] Hospital based Histology Labs
To: "Barnhart, Tammy" <Tbarnhart <@t> primecare.org>,	"Histonet (E-mail)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A61F23E937E4DA488E0C0F60093843D901E49884 <@t> ccetxm030.echristus.net>
Content-Type: text/plain;	charset="iso-8859-1"

We are located one floor above the surgical suites, but we do maintain a
frozen/gross room in the surgical suite area.  At the end of the day the
gross tech brings the specimens upstairs to be loaded in the processors.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live
free speech!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barnhart,
Tammy
Sent: Monday, May 09, 2005 9:26 AM
To: Histonet (E-mail)
Subject: [Histonet] Hospital based Histology Labs

This is a question for hospital based histo labs.  The hospital we are based
in has talked of moving our histology laboratory to an area separated from
the surgical suites.  My question is, how many hospital histology labs are
removed from the surgical suites by a significant distance?  Of those that
are separated, do you have a frozen/gross area located away from the histo
lab but close to the OR?  As many responses as possible would be
appreciated.  This will greatly facilitate our decisions for future
locations.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

Confidentiality Notice:This e-mail message is for sole use of intended
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unauthorized review, use, disclosure, distribution, or copying is
prohibited. If you are not the intended recipient, please contact the sender
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_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 8
Date: Mon, 09 May 2005 08:41:10 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Book- Immunoenzyme Multiple Staining Methods
To: Cynthia A Delong <DELONG_CYNTHIA_A <@t> LILLY.COM>,
	Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20050509083836.01b613a8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

I replaced my copy from Amazon.com.   The book is now 8.99/copy, and the 
book is no longer in print, so don't hesitate too long to replace.   I was 
so worried this would happen to me i.e. walking off, I now keep a personal 
copy at home, my first one is worn out from use!

At 08:13 AM 5/9/2005, you wrote:
>Hello All,
>I am looking for Chris Van de Loos's  book"Immunoenzyme Multiple Staining
>Methods". Does anyone know of where I can get a copy of this book?
>I had one and someone walked off with it.
>Thank you
>Cindy
>
>Cynthia A DeLong
>LRL - Corporate Center
>DC0533 - 48B/3042
>355 E Merrill St
>Indianapolis, IN  46225
>phone:  317-276-7635
>fax:  317-277-6146
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

------------------------------

Message: 9
Date: Mon,  9 May 2005 10:46:49 -0400
From: pmarcum <@t> vet.upenn.edu
Subject: Re: [Histonet] Book- Immunoenzyme Multiple Staining Methods
To: Gayle Callis <gcallis <@t> montana.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu,	Cynthia A Delong
	<DELONG_CYNTHIA_A <@t> LILLY.COM>
Message-ID: <1115650008.427f77d900571 <@t> imp.vet.upenn.edu>
Content-Type: text/plain; charset=ISO-8859-1

Hi Gayle and All,

I just purchased one from Amazon and only two were still listed at $8.95
p;us
shipping and handling it was about $12.50 for the copy.

Thanks,

Pam Marcum

Quoting Gayle Callis <gcallis <@t> montana.edu>:

> I replaced my copy from Amazon.com.   The book is now 8.99/copy, and the 
> book is no longer in print, so don't hesitate too long to replace.   I was

> so worried this would happen to me i.e. walking off, I now keep a personal

> copy at home, my first one is worn out from use!
> 
> At 08:13 AM 5/9/2005, you wrote:
> >Hello All,
> >I am looking for Chris Van de Loos's  book"Immunoenzyme Multiple Staining
> >Methods". Does anyone know of where I can get a copy of this book?
> >I had one and someone walked off with it.
> >Thank you
> >Cindy
> >
> >Cynthia A DeLong
> >LRL - Corporate Center
> >DC0533 - 48B/3042
> >355 E Merrill St
> >Indianapolis, IN  46225
> >phone:  317-276-7635
> >fax:  317-277-6146
> >_______________________________________________
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 10
Date: Mon, 9 May 2005 09:49:46 -0500
From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
Subject: RE: [Histonet] Hospital based Histology Labs
To: "Barnhart, Tammy" <Tbarnhart <@t> primecare.org>,	"Histonet
\(E-mail\)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<D6B654003615874B873E15BA680E2D22106EA938 <@t> uwhis-xchng1.hosp.wisc.edu>
Content-Type: text/plain;	charset="us-ascii"

Several years ago our surgical pathology, IHC/ISH, and renal labs were
moved to rented space in a VA hospital that's physically connected to
our University hospital.  The grossing and frozen section lab remained
in space adjacent to the ORs.  The cassetted specimens are brought over
from there every day on carts.  It seems to work okay.  If the surgeons
want to talk with a pathologist the next day about a specimen they have
to walk over to the VA to do so.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Barnhart, Tammy
Sent: Monday, May 09, 2005 9:26 AM
To: Histonet (E-mail)
Subject: [Histonet] Hospital based Histology Labs

This is a question for hospital based histo labs.  The hospital we are
based in has talked of moving our histology laboratory to an area
separated from the surgical suites.  My question is, how many hospital
histology labs are removed from the surgical suites by a significant
distance?  Of those that are separated, do you have a frozen/gross area
located away from the histo lab but close to the OR?  As many responses
as possible would be appreciated.  This will greatly facilitate our
decisions for future locations.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

Confidentiality Notice:This e-mail message is for sole use of intended
recipient(s) and may contain confidential and privileged information.
Any unauthorized review, use, disclosure, distribution, or copying is
prohibited. If you are not the intended recipient, please contact the
sender by replying to this e-mail and destroy/delete all copies of this
e-mail message. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Mon, 09 May 2005 08:59:15 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Autoflourescence Eliminator Reagent for lipofuscin
	in CNS, other cells 
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20050509084844.01b22e70 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Dear all

For those looking for a way to eliminate or reduce lipofuscin 
autofluorescence in CNS tissues and other cells, there is a commercial 
product available.  Science magazine just listed this new reagent, and it 
may be worth a try.  Chemicon International at www.chemicon.com is the 
vendor.  They mentioned monkey, human and rat neural and other tissues as 
well.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

------------------------------

Message: 12
Date: Mon, 9 May 2005 11:11:13 -0400
From: "Riesen, Rebecca" <rebecca.riesen <@t> dsilabs.com>
Subject: [Histonet] Phoenix fill in
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<05844092236E7749A1BBBCB1077C34A2DE9FDD <@t> dsi-ex01.gateway.dom>
Content-Type: text/plain;	charset="iso-8859-1"

	PICK ME! PICK ME!  Fly me to Phoenix for the weekend!  I'm in
Florida.

	Message: 10
	Date: Fri, 6 May 2005 15:30:16 -0700 (PDT)
	From: Linda Hines <linhines <@t> yahoo.com>
	Subject: [Histonet] histologist to fill in
	To: Histonet <@t> lists.utsouthwestern.edu
	Message-ID: <20050506223021.87346.qmail <@t> web31315.mail.mud.yahoo.com>
	Content-Type: text/plain; charset=us-ascii

	Hi! Everyone, I am looking for a histotech to fill in on May 20,
2005 in the afternoon appx.  4:00pm in the afternoon and work until appx.
9:00 pm. We are located central Phoenix, AZ
	if interested please contact Linda Hines @ 602-241-5134.
	Thank-you
	Linda Hines HT
	Specialty Diagnostics

	---------------------------------
	Yahoo! Mail
	 Stay connected, organized, and protected. Take the tour

	------------------------------

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Message: 13
Date: Mon, 9 May 2005 16:12:05 +0100 
From: Kemlo Rogerson <Kemlo.Rogerson <@t> elht.nhs.uk>
Subject: RE: [Histonet] Hospital based Histology Labs
To: "'Barnhart, Tammy'" <Tbarnhart <@t> primecare.org>, "Histonet (E-mail)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<1030B679AD69D6119C3F00080210DD9D05A3F28D <@t> bhrv-nt-11.bhrv.nwest.nhs.uk>

Content-Type: text/plain

Very interesting question and very pertinent for Laboratory Modernisation in
the UK. Traditionally it has always been supposed that Histology Labs must
be on Site for the very reasons you suggest. My experience in London and now
in the North leads me to question that. I accept that frozen sections may
need a local presence, but they are relatively rare nowadays, but a
motorbike Courier service may be quicker than a porter!

I think a happy intermediary is for there to be a Core Lab, that deals with
the bulk of the processing and staining and this will reduce the duplication
of tests and increase the critical number of Staff; you should see costs
driven down. ICC and such like can be consolidated on one Site again with
cost savings. A satellite laboratory may be needed to deal with frozen
sections and urgent rapid H&E's, but I wonder if that necessity, once the
Core Lab became organised may 'wither on the vine'. The bigger problems are
MDT's and alienation of Pathologists from their Clinical colleagues;
Telepathology and the use of video cameras are a solution, but not a
complete one; the spontaneous ways we humans do business makes that an
unacceptable means of communicating.

In order to release resources consolidation must occur and if used wisely
may result in extension of roles with the positive impact on recruitment and
retention of Staff.

Do you get Black Pudding in America?

-----Original Message-----
From: Barnhart, Tammy [mailto:Tbarnhart <@t> primecare.org] 
Sent: 09 May 2005 15:26
To: Histonet (E-mail)
Subject: [Histonet] Hospital based Histology Labs

This is a question for hospital based histo labs.  The hospital we are based
in has talked of moving our histology laboratory to an area separated from
the surgical suites.  My question is, how many hospital histology labs are
removed from the surgical suites by a significant distance?  Of those that
are separated, do you have a frozen/gross area located away from the histo
lab but close to the OR?  As many responses as possible would be
appreciated.  This will greatly facilitate our decisions for future
locations.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

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Message: 14
Date: Mon, 9 May 2005 11:19:03 -0400
From: <pathrm35 <@t> adelphia.net>
Subject: [Histonet] microscope companies
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<18444780.1115651943878.JavaMail.root <@t> web4.mail.adelphia.net>
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Fellow tech's,
Does anyone know of a company in Florida that services microscopes? I have
already contacted JL Optical and Southern Microscope.
Thanks,
Ron Martin

------------------------------

Message: 15
Date: Mon, 9 May 2005 11:22:49 -0400
From: John.Sheppard <@t> Health-Partners.org
Subject: [Histonet] RE: Hospital based Histology Labs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:

<OF2B4FC86D.AD7926E5-ON85256FFC.00538100-85256FFC.00547CA3 <@t> community.msft>

Content-Type: TEXT/plain; charset="US-ASCII"

We have the surgical suites located one floor above us.  The O.R. sends a
runner down with the frozens.  We keep the cryostats here in histology.  We
do get a copy of the surgury schedule, so we have an idea when about half
the frozens are comming.  It's not perfect, but it works, as long as the
runner hand delivers the frozen to someone and does not  just leave it on
the counter.

John Sheppard HT(ASCP)
The Community Hospital
Springfield, Ohio

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Message: 16
Date: Mon, 9 May 2005 10:31:14 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] Hospital based Histology Labs
To: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>,	"Barnhart,
	Tammy" <Tbarnhart <@t> primecare.org>,	"Histonet (E-mail)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A61F23E937E4DA488E0C0F60093843D90F6AA7 <@t> ccetxm030.echristus.net>
Content-Type: text/plain;	charset="iso-8859-1"

On a previous life in the alien visited town of Roswell, NM, we had an
offsite lab.  The frozen sections were handled by the pathologists at the
hospital, but the rest of the specimens were picked up by a courier twice a
day and brought to the lab for gross.  The courier will also ensure that
there was enough materials at the frozen room daily.  And the doctors were
always kind enough to let us know when their mess was too much for us to go
and clean it up.  Ever try to clean up a weeks worth of shavings in a
cryostat?  Needles to say we stop waiting for word and visited the hospital
often to keep the room nice and tidy;0)

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live
free speech!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of GUTIERREZ,
JUAN
Sent: Monday, May 09, 2005 9:36 AM
To: Barnhart, Tammy; Histonet (E-mail)
Subject: RE: [Histonet] Hospital based Histology Labs

We are located one floor above the surgical suites, but we do maintain a
frozen/gross room in the surgical suite area.  At the end of the day the
gross tech brings the specimens upstairs to be loaded in the processors.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live
free speech!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barnhart,
Tammy
Sent: Monday, May 09, 2005 9:26 AM
To: Histonet (E-mail)
Subject: [Histonet] Hospital based Histology Labs

This is a question for hospital based histo labs.  The hospital we are based
in has talked of moving our histology laboratory to an area separated from
the surgical suites.  My question is, how many hospital histology labs are
removed from the surgical suites by a significant distance?  Of those that
are separated, do you have a frozen/gross area located away from the histo
lab but close to the OR?  As many responses as possible would be
appreciated.  This will greatly facilitate our decisions for future
locations.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

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------------------------------

Message: 17
Date: Mon, 9 May 2005 11:31:13 EDT
From: RCHIOVETTI <@t> aol.com
Subject: Re: [Histonet] microscope companies
To: pathrm35 <@t> adelphia.net, histonet <@t> lists.utsouthwestern.edu
Message-ID: <86.27bbaf54.2fb0dc41 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

In a message dated 5/9/2005 8:21:22 AM US Mountain Standard Time, 
pathrm35 <@t> adelphia.net writes:

> Does anyone know of a company in Florida that services microscopes? I have

> already contacted JL Optical and Southern Microscope.
> 

Ron,

Try Micro Optics of Florida, 3941 S.W. 47th Ave., Davie, FL, 954-791-0082, <
www.microopticsfl.com>.

They are Leica dealers, but I believe their service shop also maintains and 
repairs other brands of scopes.

Cheers,

Bob

Robert (Bob) Chiovetti, Ph.D.
Independent Consultant for The Science, Technology and Industrial Sectors
132 North Elster Drive
Tucson, AZ 85710-3212 USA
Tel./Fax 520-546-4986
Member, Arizona Small Business Association - ASBA

------------------------------

Message: 18
Date: Mon, 09 May 2005 10:39:27 -0500
From: Cynthia A Delong <DELONG_CYNTHIA_A <@t> LILLY.COM>
Subject: [Histonet] Thanks Book ordered
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OF0D25B585.E9CE3623-ON05256FFC.0055EC71 <@t> EliLilly.lilly.com>
Content-Type: text/plain; charset="us-ascii"

Thanks to all who replied to my request. 
The book is ordered. 
I guess I will have to keep this one under lock and key.
Cindy

Cynthia A DeLong
LRL - Corporate Center
DC0533 - 48B/3042
355 E Merrill St
Indianapolis, IN  46225
phone:  317-276-7635
fax:  317-277-6146

------------------------------

Message: 19
Date: Mon, 9 May 2005 18:40:15 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Hospital based Histology Labs
To: "Histonetliste \(Histonetliste\)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.0.1115658000.1963.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="iso-8859-1"

Our lab is on the hospital area, but in another building as the surgery
area. We are connected with the surgery room with a pneumatic delivery, that
shots the tissue to us. That takes less than 30 sec. We do the frozens in an
integrated part of our lab. The pathologists give the results through an
intercommunication system to the surgeon. 

Gudrun Lang
Linz, Austria

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Barnhart,
Tammy
Gesendet: Montag, 09. Mai 2005 16:26
An: Histonet (E-mail)
Betreff: [Histonet] Hospital based Histology Labs

This is a question for hospital based histo labs.  The hospital we are based
in has talked of moving our histology laboratory to an area separated from
the surgical suites.  My question is, how many hospital histology labs are
removed from the surgical suites by a significant distance?  Of those that
are separated, do you have a frozen/gross area located away from the histo
lab but close to the OR?  As many responses as possible would be
appreciated.  This will greatly facilitate our decisions for future
locations.

Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND

Confidentiality Notice:This e-mail message is for sole use of intended
recipient(s) and may contain confidential and privileged information. Any
unauthorized review, use, disclosure, distribution, or copying is
prohibited. If you are not the intended recipient, please contact the sender
by replying to this e-mail and destroy/delete all copies of this e-mail
message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

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