[Histonet] TRAP on bones

[Gayle Callis]

EDTA decalcification is preferred decalcifier.  Formic acid is more than 
likely killing the enzyme, rendering it unstainable.  I have a method from 
Hermina Borgerink, if you want it.


At 10:05 AM 5/6/2005, you wrote:
>Good morning all,
>
>I have been trying to stain formic acid/EDTA decalcified formalin fixed 
>mouse femurs for osteoclasts with the TRAP stain.  I have had little 
>success.  Does anyone have a protocol that I might be able to try?  Any 
>suggestions would be greatly appreciated.  I have been using the Sigma kit 
>#387A thus far.
>
>Thanks,
>Leslie Dixon
>Mayo Clinic Scottsdale Research Histology
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)