[Histonet] Masson's trichrome

[NIDAL E MUVARAK]

I also did MTS for the first time on human saphenous vein. Got intense 
blue staining throughout the vessel and some red in the middle, 
but no nuclear staining. Here's the protocol I followed (if 
you're interested, Julien):

Overnight in Bouin's solution
Wash in tap water for 1 min
Weigert's hematoxylin for 10 min
Wash in tap water for 1 min, blue in 37mM ammonia water
Beibrich scarlet for 5 min
Wash in distilled water for 1 min
Phosphotungstic/phosphomolybdic acid for 10 min
Discard and directly add aniline blue for 5 minutes
Wash in distilled water for 1 min
Wash in acetic acid for 1 min, then again with distilled water for 1 
min
Dehydrate, clear, coverslip

Anything wrong with this protocol? 


----- Original Message -----
From: Julien LambreyDeSouza <julien_lambreydesouza <@t> uqar.qc.ca>
Date: Wednesday, May 4, 2005 12:30 pm
Subject: [Histonet] Masson's trichrome

> 
> Hello,
> 
> I am trying out Masson's trichrome for the first time. I used the 
> Goldner 
> modification from Gabe 1976, with Fuschine-Ponceau, orang-G 
> phosphomolybdic 
> and Light green. We are staining 7 micron sections of entire fish 
> larvae 
> heads. When following the protocol (5 minutes in each color 
> followed by a 
> 1% acetic water rinse) I get a general green staining of all 
> tissues. The 
> only way to differentiate cartilage from the rest in our section 
> is by 
> recognizing cellular topography, same as with Hematox-eosine 
> staining, 
> therefore loosing the whole point in using Masson's trichrome. I 
> seem to be 
> doing something wrong.
> 
> Would anybody familiar with the technique be inclined to share 
> their 
> expertise or protocol? I would be happy to send a pic of our 
> staining 
> result by email to have input on the possible problem.
> 
> Thanks,
> 
> Julien.
> Julien Lambrey de Souza
> Assistant de recherche, Biologiste M.Sc.
> Biologie Évolutive.
> Université du Québec à Rimouski
> Département de Biologie, Chimie et Sciences santé
> 300, allée des Ursulines
> Rimouski (Québec) Canada G5L 3A1
> Tél.: (418) 723-1986 ext. 1714
> Fax.: (418) 724-1849
> Courriel: julien_lambreydesouza <@t> uqar.qc.ca
> 
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