[Histonet] Masson's trichrome

[Julien LambreyDeSouza]

Hello,

I am trying out Masson's trichrome for the first time. I used the Goldner 
modification from Gabe 1976, with Fuschine-Ponceau, orang-G phosphomolybdic 
and Light green. We are staining 7 micron sections of entire fish larvae 
heads. When following the protocol (5 minutes in each color followed by a 
1% acetic water rinse) I get a general green staining of all tissues. The 
only way to differentiate cartilage from the rest in our section is by 
recognizing cellular topography, same as with Hematox-eosine staining, 
therefore loosing the whole point in using Masson's trichrome. I seem to be 
doing something wrong.

Would anybody familiar with the technique be inclined to share their 
expertise or protocol? I would be happy to send a pic of our staining 
result by email to have input on the possible problem.

Thanks,

Julien.
Julien Lambrey de Souza
Assistant de recherche, Biologiste M.Sc.
Biologie Évolutive.
Université du Québec à Rimouski
Département de Biologie, Chimie et Sciences santé
300, allée des Ursulines
Rimouski (Québec) Canada G5L 3A1
Tél.: (418) 723-1986 ext. 1714
Fax.: (418) 724-1849
Courriel: julien_lambreydesouza <@t> uqar.qc.ca