[Histonet] cutting angle

[Roxanne Soto]

The microtome is an olympus.  They use an infiltrating paraffin and a different one for embedding.  The blades are accuedge.
  ----- Original Message ----- 
  From: pmarcum <@t> vet.upenn.edu<mailto:pmarcum <@t> vet.upenn.edu> 
  To: Roxanne Soto<mailto:godsgirlnow <@t> msn.com> 
  Sent: Tuesday, May 03, 2005 2:03 PM
  Subject: Re: [Histonet] cutting angle


  Roxanne,

  Did the model of the microtome change?  I find I had to change the angle when I
  used a different brand of microtome.  I also have to decide for myself what
  knife angle is best for me on a given microtome.  I dont think there is one
  absolute knife angle for all microtomes or blades.  I am currently using a
  Leica 2255 and use 3 to 5 for tungsten carbide D profile and 5 to 6 for low
  profile knife holder and blades.  I am using Thermo Shandon Premier S35 low
  profile knives for paraffin.  They are working well for me. 

  Pam Marcum

  Quoting Roxanne Soto <godsgirlnow <@t> msn.com<mailto:godsgirlnow <@t> msn.com>>:

  > What angles does everyone out there cut at and what kind of blades do you 
  > use?  I have always cut at 10 with high or low profile blades, but I have 
  > recently changed jobs and they cut at an angle of 21.  They were told that 
  > with high profile blades they should use an angle of 21.  I am having a hard
  > 
  > time cutting at this angle.......
  > any thoughts?
  > Roxanne
  > 
  > 
  > 
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